CNS MEDIATION OF THE AIRWAY RESPONSE TO LUNG INFLATION

中枢神经系统对肺充气气道反应的调节

• 批准号: 3474167
• 负责人: JAMES R HASELTON
• 金额: $ 9.58万
• 依托单位: UNIVERSITY OF NEBRASKA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-09-30 至 1996-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3474167
• 关键词: agglutinins; antidromic impulse; brain stem; bronchomotion; cats; central nervous system; central neural pathway /tract; innervation; lung; motor neurons; muscle tension; muscle tone; neural transmission; neurochemistry; neurophysiology; parabrachial nucleus; phrenic nerve; preganglionic fiber; respiratory function; respiratory muscles; respiratory reflex; single cell analysis; smooth muscle; vagus nerve

A great deaf is known about the afferent and efferent pathways responsible for mediating the Hering-Breuer Inflation reflex, but very little is known about the central nervous system pathways that are involved in this reflex arc. The influence of the Hering-Breuer Inflation reflex on respiratory rhythmogenesis and cardiovascular control has been studied extensively, while the influence of this reflex on airway tone has largely been ignored. The proposed studies are designed to elucidate the central pathways mediating the airway response to the Hering-Breuer Inflation reflex. Three central pathways, which have been documented as being significant in central respiratory control, will be examined during the course of the proposed studies. These pathways will be studied using g chemical stimulation of neuronal cell bodies to determine if the activation of potential relay sites reduces airway tone in a manner which mimics the activation of the Hering-Breuer reflex. Synaptic transmission will be blocked transiently at potential relay sites to determine if this maneuver attenuates or prevents the reflex airway response to lung inflation. Anatomical tracers will be used to confirm the participation of these potential relay sites in the Hering-Breuer reflex arc. Extracellular single-cell recording will be used in conjunction with antidromic stimulation of vagal branches that innervate the airway to permit the characterization of the spontaneous discharge pattern(s) of vagal tracheomotor and bronchomotor preganglionic neurons. These recordings will also permit the characterization of discharge response of these vagal preganglionic neurons to lung inflation before and during the blockade of synaptic transmission at the potential relay sites.

一个严重的耳聋是已知的传入和传出途径负责 调节赫林-布鲁尔膨胀反射，但知之甚少 关于中枢神经系统中参与这种反射的通路 弧 Hering-Breuer充气反射对呼吸的影响 节律发生和心血管控制已经被广泛研究， 而这种反射对气道张力的影响在很大程度上被忽略了。 拟议的研究旨在阐明中枢通路 介导对Hering-Breuer充气反射的气道反应。 三 中央通路，这已被证明是重要的， 中央呼吸控制，将在过程中进行检查， 建议的研究。 这些途径将研究使用g化学 刺激神经元细胞体，以确定是否激活 潜在的中继部位以模仿呼吸道紧张的方式降低气道紧张， Hering-Breuer反射 突触传递将是 在潜在的中继站短暂受阻，以确定这一动作是否 减弱或防止对肺充气的反射气道反应。 解剖示踪剂将用于确认这些参与 黑-布反射弧中的潜在中继点 细胞外 单细胞记录将与逆向 刺激支配气道的迷走神经分支， 迷走神经的自发放电模式的表征 气管和支气管节前神经元。 这些录音将 也允许表征这些迷走神经的放电反应 在阻断前和阻断期间， 突触传递在潜在的中继站点。