ELECTROPHYSIOLOGY OF DENTATE GYRUS

齿状回提供主要输入到海马适当通过 贯穿的路径，并已牵连在某些发病机制 人类和动物癫痫的类型。 然而，电生理 在该区域发现的细胞的特征知之甚少。 该建议旨在检查电生理学特性， 人类和啮齿动物齿状回的细胞。 在这个实验中使用的人体组织 一项研究是从难治性癫痫患者身上取活检组织 福尔斯分为两类： 海马体中的一些生化和组织学标记 受到影响，而那些在颞叶有病变或肿瘤的人， 这些标记相对不受影响。 所有实验 将在啮齿动物组织中平行进行。 的 在体外记录中， 室和电生理记录和细胞标记技术 将用于 该项目的主要目标是确定是否有具体的变化， 单细胞水平，可以在癫痫组织中看到， 比较人类组织和啮齿动物对照组。 我们的具体目标 包括：第一，将形态学和电生理学分类 颗粒细胞和中间神经元的特征， 对两种类型的人体组织以及啮齿动物都有齿;第二， 研究兴奋和抑制特性及其调制， 这三种类型的组织和第三，以评估行动的一些 肽类神经递质会影响颞叶 硬化症 该提案的很大一部分用于研究 NMDA型谷氨酸受体的调节，在三类 组织. 这种受体似乎是癫痫样活动的基础。 动物癫痫模型的数量，从而改变这种调制 受体亚型的候选人的过度兴奋中看到， 表皮组织 将详细研究三种调制器： 胞外pH、Zn和Mg。

The dentate gyrus provides the primary input to the hippocampus proper via the perforant path and has been implicated in the pathogenesis of certain types of human and animal epilepsy. However, the electrophysiological characteristics of the cells found in this region are poorly understood. This proposal is designed to examine the electrophysiological properties of cells from human and rodent dentate gyri. The human tissue used in this study is biopsy tissue from patients with medically intractable epilepsy and falls into two categories: those with medial temporal lobe sclerosis in which a number of biochemical and histological markers in the hippocampus are affected, and those with lesions or tumors in the temporal lobe in which these markers are relatively unaffected. All the experiments outlined below will be performed in parallel in rodent tissue. The hippocampi are maintained as 400 micrometer slices in an in vitro recording chamber and electrophysiological recording and cell labelling techniques will be used. The broad goals of this project are to determine if specific changes, at the level of single cells, can be seen in epileptic tissue relative to the comparison human tissue and to rodent controls. Our specific goals include: first, to classify the morphological and electrophysiological characteristics of both the granule cells and interneurons found in the dentate for the two types of human tissue as well as for rodents; second, to study the exciatory and inhibitory properties and their modulation in these three types of tissue and third to assess the actions of a number of peptide neurotransmitters which are affected in cases of temporal lobe sclerosis. A significant portion of this proposal is devoted to studying the modulation of the NMDA type glutamate receptor in the three classes of tissue. This receptor appears to underlie epileptiform activity in a number of animal epilepsy models and thus changes in the modulation of this receptor subtype are candidates for the hyperexcitability seen in the sclerotic tissue. Three modulators will be examined in detail: extracellular pH, Zn, and Mg.