NEUROENDOCRINE CONTROL OF ANTERIOR PITUITARY HORMONES
垂体前叶激素的神经内分泌控制
基本信息
- 批准号:3483569
- 负责人:
- 金额:$ 4.31万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1981
- 资助国家:美国
- 起止时间:1981-07-01 至 1992-08-31
- 项目状态:已结题
- 来源:
- 关键词:acromegaly alpha methyldopamine blood chemistry catecholamine inhibitor dopamine endocrine disorder endocrine pharmacology estrogens hormone receptor hormone regulation /control mechanism human subject hyperprolactinemia insulinlike growth factor laboratory rat neural information processing neuroendocrine system neuropeptides orphan disease /drug peptide hormone metabolism pituitary disorders pituitary hormones pituitary neoplasms prolactin prolactin releasing /inhibiting factor radioimmunoassay somatostatin somatotropin thyroid hormones tissue /cell culture
项目摘要
The goals of this proposal are to study the regulation of growth
hormone (GH) secretion, the hypothalamic releasing and inhibiting
hormones (GH-releasing hormone (GRH) and somatostatin (SRIF)) that
control the secretion of GH, and to search for alterations in
neuroendocrine function that may be responsible for disorders of
GH secretion in man. The identification of each of the component
hormones in this regulatory system (GRH, SRIF, GH, and insulin-like
growth factor-l (IGF-l), the mediator of many of the growth
promoting action of GH) and their molecular cloning allows for an
integrated approach to the study of this hypothalamic-pituitary
system that may serve as a model for other systems. The proposal
will utilize techniques of molecular biology, cell culture, protein
chemistry, in vivo measurements of hormone levels in hypophyseal
portal blood, and clinical studies to study different aspects of
this system. The regulation of hypothalamic GRH gene expression
will be studied in vivo in response to hormonal and metabolic
(starvation, diabetes, obesity) perturbations, and in vitro using
GRH gene transfection. The conversion of the GRH precursor to the
mature hormone will be studied in transgenic animals expressing
the human GRH gene and the role of processing enzymes as potential
regulators will be determined. The metabolism of GRH will be
studied in circulation and at the sites of GRH action and the
enzymes involved will be characterized. The neuroendocrine
regulation of GRH and SRIF will be studied by direct measurements
of the two neurohormones in portal plasma and in hypothalamic
perfusate fluid of unanesthetized sheep and the molecular forms of
each hormone will be determined by high performance liquid
chromatography. GRH secretion from GRH-containing neuronal cells
in culture will be studied and the effects of secretogogues
determined. GRH and SRIF action on the pituitary will be studied
in the GH deficient lit/lit mouse and membrane fusion will be
performed in an attempt to correct the previously reported
abnormality presumed to reside in the GRH receptor. Our newly
developed somatomammotroph cell line, the first that exhibits GRH
sensitivity, will be used to study the effects of GRH and SRIF at
cell membrane and nuclear levels. A comparison of exogenous and
endogenous (by transfection of the GRH gene) GRH effects will also
be compared. The molecular structure of circulating GRH in human
plasma will be determined and the mechanism of GH feedback effects
in normals and in subjects with disorders of GH secretion will be
assessed. The results of these studies will provide a better
understanding of the role of the central nervous system in clinical
disorders of GH secretion and may ultimately lead to new
therapeutic approaches.
本提案的目标是研究增长调控
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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LAWRENCE A FROHMAN其他文献
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{{ truncateString('LAWRENCE A FROHMAN', 18)}}的其他基金
NEUROENDOCRINE CONTROL OF ANTERIOR PITUITARY HORMONES
垂体前叶激素的神经内分泌控制
- 批准号:
3483570 - 财政年份:1981
- 资助金额:
$ 4.31万 - 项目类别:
NEUROENDOCRINE CONTROL OF ANTERIOR PITUITARY HORMONES
垂体前叶激素的神经内分泌控制
- 批准号:
3483567 - 财政年份:1981
- 资助金额:
$ 4.31万 - 项目类别:
NEUROENDOCRINE CONTROL OF ANTERIOR PITUITARY HORMONES
垂体前叶激素的神经内分泌控制
- 批准号:
3483566 - 财政年份:1981
- 资助金额:
$ 4.31万 - 项目类别:
NEUROENDOCRINE CONTROL OF ANTERIOR PITUITARY HORMONES
垂体前叶激素的神经内分泌控制
- 批准号:
3152101 - 财政年份:1981
- 资助金额:
$ 4.31万 - 项目类别:
NEUROENDOCRINE CONTROL OF ANTERIOR PITUITARY HORMONES
垂体前叶激素的神经内分泌控制
- 批准号:
3483568 - 财政年份:1981
- 资助金额:
$ 4.31万 - 项目类别:
NEUROENDOCRINE CONTROL OF ANTERIOR PITUITARY HORMONES
垂体前叶激素的神经内分泌控制
- 批准号:
2138483 - 财政年份:1981
- 资助金额:
$ 4.31万 - 项目类别:
NEUROENDOCRINE CONTROL OF ANTERIOR PITUITARY HORMONES
垂体前叶激素的神经内分泌控制
- 批准号:
2138482 - 财政年份:1981
- 资助金额:
$ 4.31万 - 项目类别:
NEUROENDOCRINE CONTROL OF ANTERIOR PITUITARY HORMONES
垂体前叶激素的神经内分泌控制
- 批准号:
3229580 - 财政年份:1981
- 资助金额:
$ 4.31万 - 项目类别:
NEUROENDOCRINE CONTROL OF ANTERIOR PITUITARY HORMONES
垂体前叶激素的神经内分泌控制
- 批准号:
3229582 - 财政年份:1981
- 资助金额:
$ 4.31万 - 项目类别:
NEUROENDOCRINE CONTROL OF ANTERIOR PITUITARY HORMONES
垂体前叶激素的神经内分泌控制
- 批准号:
3229581 - 财政年份:1981
- 资助金额:
$ 4.31万 - 项目类别: