RAPID BIOSENSOR TEST FOR HEPB CORE ANTIGENS ANTIBODIES

这项研究的长期目标是开发一种快速， 一种经济有效的检测人抗B型肝炎核心抗体的方法 抗原（HepBcA），适用于献血前 筛选献血者，并将利用新的电容 亲和传感器技术 本研究的具体目的 主要有：1）评价HepBcA的固定化方法 在生物传感器的表面上，2）评估人血清 蛋白质作为参比生物传感器的涂层，以及3）确定 电容的大小和特异性改变， HepBcA和人血清蛋白包被的生物传感器相互作用 与抗HepBcA的小鼠单克隆抗体和人抗体。 该项目将通过以下方式促进技术创新： 评估电容亲和传感器技术的适用性 血库目前面临的一个主要问题： 检测HepBcA抗体，替代检测非A，非 B型肝炎。 开发适用于以下人群的HepBcA抗体检测 捐赠者的捐赠前筛查应该有大量的 商业潜力，无论是在美国和几个外国 国家

The long-term objective of this research is to develop a rapid, cost-effective test for human antibodies to Hepatitis B core antigen (HepBcA) which would be suitable for predonation screening of blood donors, and would utilize novel capacitive affinity sensor technology. The specific aims of this research are: 1) to evaluate methods by which HepBcA can be immobilized on the surface of the biosensor, 2) to evaluate a human serum protein as a coating for a reference biosensor, and 3) to determine the magnitude and specificity of the capacitance changes when HepBcA- and human serum protein-coated biosensors interact with mouse monoclonal and human antibodies against HepBcA. This project will contribute to technological innovation by assessing the applicability of capacitive affinity sensor technology to a major current problem for blood banks: the high cost of testing for antibodies to HepBcA, a surrogate test for non-A, non- B hepatitis. Developing a HepBcA antibody test suitable for predonation screening of donors should have substantial commercial potential, both in the U.S. and in several foreign countries.