CELL SECRETION AND MEMBRANE FORMATION IN THE PANCREAS
胰腺中的细胞分泌和膜形成
基本信息
- 批准号:3483203
- 负责人:
- 金额:$ 25.03万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1977
- 资助国家:美国
- 起止时间:1977-08-01 至 1992-07-31
- 项目状态:已结题
- 来源:
- 关键词:acinar cell affinity chromatography apical membrane autoradiography basolateral membrane binding proteins cell cell interaction cell differentiation cell membrane complementary DNA electron microscopy embryo /fetus cell /tissue enzyme mechanism glycoproteins hormone binding protein hormone receptor immunochemistry intracellular transport laboratory mouse laboratory rat membrane proteins membrane reconstitution /synthesis nucleic acid probes pancreas phosphorylation pinocytosis protein transport radiotracer scanning electron microscopy secretion secretory protein surface property tissue /cell culture zymogens
项目摘要
Polarized secretory epithelial cells possess several sorting devices
to effect vectorial flow of secretory and membrane proteins to
their apical and basolateral poles. Our previous studies on
pancreatic acinar cells have delineated the kinetics and routes of
intracellular transpost of secretory proteins that enter a
secretagogue activated (regulated) pathway leading to apical
exocytosis while a separate secretory pathway results in
basolateral, nonstimulated (constitutive) discharge of basement
membrane proteins. Similarly, vectorial delivery of membrane
proteins to the apical and basolateral poles of secretory cells is
required to establish and maintain structural and functional
poolarity. The goal of this project is to define the routes and
control mechanisms for constitutive and regulated secretory
pathways in pancreatic acinar and other epithelial cells and to
characterize the pathways responsible for biogenesis of
plasmalemmal polarity. To this end, we will use pancreatic acinar
and other cell lines cultured in chambers that allow probing of
apical and basolateral secretory compartments. Agents that
perturb secretory protein sorting will be tested for their ability to
affect differentially apical (e.g. amylase) or basolateral (e.g.
basal lamina) secretory pathways. These will include
secretagogues, acidothrophic agents, inhibitors of glycosylation
and protein synthesis and drugs producing cytoskeletal
disassembly. The effects of reduction in temperature and ATP
levels will also be examined. Comparable experimental conditions
will be used, in conjunction with immnoassays for polarity of
apical (gamma-glutamyl transferase) and basolateral (insulin and
laminin receptors) membrane protein delivery, to define factors
regulating biogenesis of plasmalemmal polarity and to clarify
relationships between secretory protein and membrane protein
delivery pathways. We will also examine membrane targeting in
MDCX cells transfected with cDNAs for gamma-glutamyl
transferase, an apical and basolateral membrane protein in
pancreatic acinar cells. Finally, we plan to study biogenesis of
the regulated secretory pathway in developing pancreas as it
acquires secretagogue responsiveness.
极化分泌上皮细胞具有几种分选装置
以影响分泌和膜蛋白的矢量流,
它们的顶极和基底极。 我们以前的研究
胰腺腺泡细胞已经描绘了
分泌性蛋白质的细胞内转运,
促分泌素激活(调节)途径导致顶端
胞吐作用,而单独的分泌途径导致
基底外侧非刺激性(组成性)基底放电
膜蛋白 类似地,膜的载体递送
蛋白质的顶端和基底外侧极的分泌细胞是
需要建立和维护结构和功能
池性 该项目的目标是确定路线,
组成性和调节性分泌的控制机制
胰腺腺泡和其他上皮细胞中的信号通路,
表征负责生物起源的途径
质膜极性 为此,我们将使用胰腺腺泡
和其他细胞系培养在小室中,
顶端和基底外侧分泌室。 的试剂
干扰分泌蛋白分选将测试它们的能力,
影响差异顶端(如淀粉酶)或基底侧(如
基底层)分泌途径。 这些将包括
促分泌剂、促酸剂、糖基化抑制剂
蛋白质合成和药物生产细胞骨架
拆卸 温度和ATP降低的影响
还将检查水平。 可比实验条件
将与免疫测定结合使用,以确定
顶侧(γ-谷氨酰转移酶)和底侧(胰岛素和
层粘连蛋白受体)膜蛋白递送,以确定因子
调节质膜极性的生物发生,
分泌蛋白与膜蛋白的关系
传递途径。 我们还将研究膜靶向在
用γ-谷氨酰cDNA转染的MDCX细胞
转移酶,一种顶侧和基底侧膜蛋白,
胰腺腺泡细胞 最后,我们计划研究
在发育中的胰腺中调节分泌途径,
获得促分泌素反应性。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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JAMES Douglas JAMIESON其他文献
JAMES Douglas JAMIESON的其他文献
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{{ truncateString('JAMES Douglas JAMIESON', 18)}}的其他基金
WORKSHOP ON INTRACELLULAR PROTEIN IN SECRETORY CELLS
分泌细胞内蛋白质研讨会
- 批准号:
3434672 - 财政年份:1990
- 资助金额:
$ 25.03万 - 项目类别:
MEMBRANE PROTEIN TRAFFIC IN NORMAL AND TRANSFORMED CELLS
正常细胞和转化细胞中的膜蛋白运输
- 批准号:
3094195 - 财政年份:1988
- 资助金额:
$ 25.03万 - 项目类别:
CELL SECRETION AND MEMBRANE FORMATION IN THE PANCREAS
胰腺中的细胞分泌和膜形成
- 批准号:
2749420 - 财政年份:1977
- 资助金额:
$ 25.03万 - 项目类别:
CELL SECRETION AND MEMBRANE FORMATION IN THE PANCREAS
胰腺中的细胞分泌和膜形成
- 批准号:
2137076 - 财政年份:1977
- 资助金额:
$ 25.03万 - 项目类别:
CELL SECRETIN AND MEMBRANE FORMATION IN THE PANCREAS
胰腺细胞分泌素和膜形成
- 批准号:
2137074 - 财政年份:1977
- 资助金额:
$ 25.03万 - 项目类别:
CELL SECRETION AND MEMBRANE FORMATION IN THE PANCREAS
胰腺中的细胞分泌和膜形成
- 批准号:
3483202 - 财政年份:1977
- 资助金额:
$ 25.03万 - 项目类别:
CELL SECRETIN AND MEMBRANE FORMATION IN THE PANCREAS
胰腺细胞分泌素和膜形成
- 批准号:
2137075 - 财政年份:1977
- 资助金额:
$ 25.03万 - 项目类别:
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