ELECTROFUSION AND ELECTROPERMEATION OF CELLS

细胞的电融合和电渗透

• 批准号: 3497871
• 负责人: HILDA LIANG
• 金额: $ 4.84万
• 依托单位: ELECTROCELL
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-04-01 至 1987-12-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3497871
• 关键词: cell membrane; cell type; computer system design /evaluation; drug delivery systems; hybrid cells; hybridomas; liposomes; membrane fusion; membrane structure; monoclonal antibody

The technique of electroporation is utilized to permeate cell membranes so as to introduce drugs or foreign genetic materials into living cells. Electrofusion is used as a new approach to form hybrid cells for genetics studies and monoclonal antibody production, as well as for drug infusion via liposomes. We plan to develop protocols for electroporation and electrofusion of common cell types, based on our understanding of the physical properties of cells. Prototypes of electronic cell fuser and porator will be designed according to our protocols. Microscopic, macroscopic and continuous-flow cell fusion chambers will be made. The successful development of both proprietary hardwares and softwares (procedures and formulae) will form a basis for commercialization . The Buffalo biomedical community provides an excellent environment for biotechnology transfer and feedback, as well as a test market for future expansion.

利用电穿孔技术来渗透电池 用于导入药物或外来遗传物质的膜 变成活细胞。电融合被用作一种新的形成方式 用于遗传学研究的杂交细胞和单抗 生产，以及通过脂质体进行药物输注。我们计划 开发用于电穿孔和电融合的方案 常见的细胞类型，基于我们对物理的理解 单元的属性。电子电池熔断器样机和 传送器将根据我们的协议进行设计。微观的， 宏观和连续流动的细胞融合室将是 制造。这两款专有硬件的成功开发 和软件(程序和公式)将构成 商业化。布法罗生物医学社区提供 良好的生物技术转让环境和 反馈，以及未来扩张的测试市场。