ISOLATION OF ALBUMIN BY CONTINUOUS AFFINITY SEPARATION

拟议的工作是为了开发一部小说和 一种高效的连续分离纯化方法 来自复杂和稀薄混合物的生物分子。最近的事态发展 生物学允许设计和培养微生物和能够 生产临床或商业用的特定蛋白质或酶的 重要性。这些蛋白质产品的生产包括两个步骤： 1)发酵或细胞培养；2)分离纯化。 尽管发酵和细胞培养最近经历了很长时间 开发、分离和纯化仍然是最薄弱的部分 整体流程。 第一阶段计划的目标是确定以下项目的可行性 将中空纤维膜与亲和结合用于 蛋白质的提纯。我们已经确定了移除和隔离 从人血浆中提取白蛋白作为一个有价值的项目来评估 我们方法的可行性。我们期待在这个项目中取得成功 将为其他商品的提纯提供一种通用的方法 重要的蛋白质。

The proposed work is directed toward the development of a novel and efficient method for the continuous isolation and purification of biomolecules from complex and dilute mixtures. Recent developments in biology allow the design and culture of micro-organisms and cells capable of producing specific proteins or enzymes of clinical or commercial importance. Production of these protein products consists of two steps: 1) fermentation or cell culture, and 2) isolation and purification. Although fermentation and cell culture have undergone much recent development, isolation and purification remain the weakest part of the overall process. The goal of the Phase I program is to establish the feasibility of employing hollow-fiber membranes in conjunction with affinity binding for the purification of proteins. We have identified the removal and isolation of albumin from human plasma as a worthwhile project to evaluate the feasibility of our approach. We anticipate that success in this program will offer a general method for the purification of other commercially important proteins.