STRUCTURE AND FUNCTION OF PEP CARBOXYKINASE ISOZYMES

研究计划的目标是研究结构，合成和 线粒体和胞质磷酸烯醇式丙酮酸的加工 羧酸激酶。这将包括对酶蛋白进行测序以及 利用基因工程技术克隆磷酸烯醇式丙酮酸羧基激酶基因的全长cDNA 重组DNA程序。关于酶的结构的信息 将使我们更好地了解它的功能，特别是它的调节 受金属离子(Mn2、Fe2)等辅助因素的影响。磷酸烯醇式丙酮酸 羧酸激酶是独一无二的，因为在大多数物种中，它都存在于 胞浆和线粒体。大豆蛋白的合成、运输和加工 将研究线粒体酶，并将其与胞浆同工酶进行比较 为了更好地了解基因表达的协调调控。 由于磷酸烯醇式丙酮酸羧激酶是一种关键的起搏酶，因此在 肝脏和肾脏的糖异生，我们的发现将有助于 更好地理解脊椎动物葡萄糖稳态的调节 并允许我们类比对这两种形式的 酶在糖尿病等疾病过程中的作用。

The goal of the research program is to study the structure, synthesis and processing of mitochondrial and cytosolic phosphoenolpyruvate carboxykinase. This will involve sequencing the enzyme protein as well as cloning a cDNA to the phosphoenolpyruvate carboxykinase mRNA using recombinant DNA procedures. Information on the structure of the enzyme will allow us to better understand its function, expecially its regulation by cofactors such as metal ions (Mn2+, Fe2+). Phosphoenolpyruvate carboxykinase is unique since, in most species, it is present in both the cytosol and mitochondria. The synthesis, transport and processing of the mitochondrial enzyme will be studied and compared to the cytosol isozyme in order to better understand the coordinate regulation of gene expression. Since phosphoenolpyruvate carboxykinase is a key, pacesetting enzyme in both hepatic and renal gluconeogenesis, our findings will contribute to a better understanding of the regulation of glucose homeostasis in verebrates and allow us to draw analogies to the regulation of both forms of the enzyme in disease processes such as diabetes.