SURGICAL STUDIES ON METABOLISM OF G I HORMONES

G I 激素代谢的外科研究

• 批准号: 3483092
• 负责人: JAMES C THOMPSON
• 金额: $ 24.06万
• 依托单位: UNIVERSITY OF TEXAS MED BR GALVESTON
• 依托单位国家: 美国
• 财政年份: 1976
• 资助国家: 美国
• 起止时间: 1976-05-01 至 1991-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3483092
• 关键词: Zollinger Ellison syndrome; bile; birth; calcium; cholecystokinin; cyclic nucleoside monophosphate; diet; digestion; esophagogastric junction; fasting; gallbladder; gastric inhibitory peptide; gastric juice; gastric mucosa; gastrointestinal hormones; gastrointestinal motility /pressure; gastrointestinal pharmacology; gastrointestinal system; glucagon; high performance liquid chromatography; hormone regulation /control mechanism; human subject; indomethacin; insulin; intestine neoplasm; liver metabolism; muscle contraction; neoplastic growth; neurotensin; nutrition related tag; pancreas disorder; pancreas enzyme; pancreas neoplasms; pancreatic polypeptide; peptic ulcer; peptide hormone; prostaglandins; radioimmunoassay; radiotracer; secretion; stomach surgery; thyroid disorder; thyroid neoplasm; tissue /cell culture; vagotomy

The objectives of this proposal are to investigate the role of gastrointestinal hormones (GIH) in the digestive (and related) processes. We will study the mechanisms for release of neurotensin (NT) from the gut and from medullary carcinoma of the thyroid. We will determine the effect of NT on gastric secretion stimulated by different routes. We plan to characterize cholecystokinin (CCK), NT, bombesin, and peptide YY (PYY) by HPLC. We will determine the differential hepatic uptake of different molecular forms of endogenously released CCK by bioassay and radioimmunoassay. We plan to study the mechanisms of actions of CCK on gallbladder (GB) smooth muscle. We will study the action of PYY on gastric secretion and measure PYY receptors in antrum and fundus. We plan to study the mechanism of relaxation of the sphincter of Oddi by CCK and to determine, by receptor studies, whether substance P is involved. We will determine the relationships between the gut and pancreatic acinus and note particularly the roles of NT, prostaglandins, and indomethacin. We will use whole animals, isolated pancreas, and dispersed acini. We plan to characterize the enterinsulinar relationships of GIH and calcium-regulatory peptides on secretion of insulin and glucagon, to determine whether intracerebroventricular administration of GI and calcium-regulatory peptides influence secretion of insulin and glucagon, and to examine the influence of pregnancy on the relationship between gut hormones and pancreatic endocrine hormone secretion. We will study the role of GIH in the inhibition and stimulation of growth of the pancreas and the relationship with hormone receptors and efficacy of action. We will study the role of stimulatory and inhibitory peptides in the pathogenesis and course of experimental acute pancreatitis. We plan to determine the effects of GIH on the stimulation and inhibition of growth of cancers of the gut and pancreas. We will measure GIH receptors in gut and pancreatic cancer cells. We plan clinical studies on the relationship between GIH and chronic pancreatitis in patients before and after operation. We will continue our metabolic studies on patients with the Zollinger-Ellison syndrome (ZES) and plan to compare nutrition after total gastrectomy in ZES and non-ZES patients. We will study the role of GIH in patients with thryoid disease. We will study the metabolism of NT in man. We will determine the effects of various stages of pregnancy, as compared with the postpartum state, on the relationship between CCK and GB contraction in women.

本提案的目的是调查以下方面的作用： 胃肠激素（GIH）在消化（及相关）过程中的作用。 我们将研究神经降压素（NT）从肠道释放的机制 以及甲状腺髓样癌。 我们将确定 NT对不同途径刺激胃分泌的影响。 我们计划 通过以下方法表征胆囊收缩素（CCK）、NT、蛙皮素和肽YY（PYY）： 高效液相色谱法 我们将确定不同的肝脏摄取差异 通过生物测定内源性释放CCK的分子形式， 放射免疫法 我们计划研究CCK的作用机制， 胆囊（GB）平滑肌。 我们将研究PYY对胃粘膜的作用， 分泌和测量胃窦和胃底的PYY受体。 我们计划学习 CCK松弛Oddi括约肌的机制及CCK对Oddi括约肌的影响 通过受体研究确定P物质是否参与其中。 我们将 确定肠道和胰腺腺泡之间的关系，并注意 特别是NT、三尖杉酯碱和吲哚美辛的作用。 我们将 使用整个动物、分离的胰腺和分散的腺泡。 我们计划 描述GIH和钙调节的肠胰岛素关系 肽对胰岛素和胰高血糖素分泌的影响，以确定是否 脑室内胃肠道给药和钙调节 肽影响胰岛素和胰高血糖素的分泌，并检查 妊娠对肠道激素与 胰腺内分泌激素分泌。 我们将研究GIH的作用， 抑制和刺激胰腺生长， 与激素受体的关系和作用效果。 我们将研究 刺激和抑制肽在发病机制中的作用， 实验性急性胰腺炎的过程。 我们计划确定 GIH对刺激和抑制肿瘤生长的作用 肠道和胰腺 我们将测量肠道和胰腺中的GIH受体， 癌细胞 我们计划对胃肠道出血与 慢性胰腺炎患者手术前后。 我们将 继续我们对Zollinger-Ellison综合征患者的代谢研究 ZES综合征患者全胃切除术后营养状况比较 非ZES患者。 我们将研究GIH在以下患者中的作用： 甲状腺疾病 我们将研究NT在人体内的代谢。我们将 确定怀孕的各个阶段的影响，与 产后状态下胆囊收缩素与胆囊收缩的关系 妇女