DEVELOPMENT OF ANTI-IDIOTYPE ANTIBODY VACCINES FOR AIDS

这项提议的长期目标是开发一种疫苗 对于艾滋病使用抗独特型抗体作为基础。要实现 这一目标在第一阶段提出了以下任务：1) 用抗HIV蛋白单抗免疫小鼠 能够在体外中和病毒的生长，并选择 产生抗独特型抗体的杂交克隆，2) 用抗独特型单抗免疫动物并随后进行试验 用于产生对艾滋病毒蛋白的免疫反应，以及 3)确定血清(抗独特型抗体)是否来自 免疫的动物能够中和艾滋病毒在体内的生长 体外系统。 这些结果将决定利用反病毒技术的可行性。 针对非法中和反应的独特型抗体 艾滋病毒，此外还将为发展 一种有效的艾滋病疫苗。

The long range objective of the proposal is to develop a vaccine for AIDS using as a basis anti-idiotypic antibodies. To achieve this goal the following tasks are proposed in Phase I: 1) to immunize mice with anti-HIV protein monoclonal antibody (MAb) which are capable of neutralizing viral growth in vitro and to select for hybrid clones which are producing anti-idiotypic antibody, 2) to immunize animals with the anti-idiotypic MAb and subsequently test for the generation of an immune response against HIV proteins, and 3) to determine if sera (anti-anti-idiotypic antibody) from immunized animals are capable of neutralizing HIV growth in an in vitro system. These results will determine the feasibility of utilizing anti- idiotypic antibodies to illicit a neutralizing response against HIV, and additionally will provide a basis for the development of an efficacious vaccine against AIDS.