DISTRIBUTION OF A CARDIOEXCITATORY NEUROPEPTIDE
心脏兴奋性神经肽的分布
基本信息
- 批准号:3485933
- 负责人:
- 金额:$ 12.18万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1981
- 资助国家:美国
- 起止时间:1981-06-01 至 1992-03-31
- 项目状态:已结题
- 来源:
- 关键词:Bivalvia Crocodilia Echinodermata Gastropoda Mollusca Tunicata amides aminoacid analyzer aquatic biology atrial natriuretic peptide cardiotonic agents chemical structure function fish ganglions heart pharmacology high performance liquid chromatography immunochemistry laboratory rabbit laboratory rat neurons neuropeptides neurotransmitter receptor radioimmunoassay radiotracer vascular smooth muscle
项目摘要
The molluscan cardioexcitatory peptide FMRFamide (Phe-Met-
Arg-Phe-NH2), together with four structurally similar peptides,
constitute a closely related nuclear family which now includes
among its membership two tetrapeptides and three heptapeptides.
The tetra-and heptapeptides have different phyletic distributions
among the molluscs, and have distinctive effects and receptors on
muscles and nerves. Antisera to FMRFamide also cross-react
widely with antigens in the nerve and endocrine cells of non-
molluscan groups, both vertebrate and invertebrate. Some of this
immunoreactivity is due to established peptides, but previously
unknown peptides have also been discovered. These
immunochemical results are manifestations of a large,
heterogeneous, widespread, extended peptide family; it is
characterized by an amidated C-terminal dipeptide of the form
Arg-X-NH2 (where X is an aromatic residue). The first long-term
goal of this project is to examine the tissue distributions, actions
and physiological roles of the individual FMRFamide-related
peptides (FaRPs) making up the model nuclear family in the
pulmonate snail, Helix aspersa. The receptors complementary to
the FaRPs will be characterized by studying the structure-
activity relations (SAR) of these peptides on whole tissues,
biochemical mechanisms, and radioligand-receptor binding
properties. The variation in the levels and potency of the FaRPs,
as a function of the activity state of the snails will also be
examined. The second long-term goal is to determine the phyletic
distribution, size, and limits of the extended family of FaRPs, and
to define its relationship with the nuclear family in molluscs.
New extra-molluscan FaRPs will be sought in central nervous
extracts of vertebrates and key invertebrates. The effects, of
both the nuclear and extended FaRPs, on vertebrate vascular
smooth muscle will be tested and the receptors characterized by
SAR. The relationship between the nuclear family of FaRPs and
the extended family may reside in the homology of their
receptors; the similarities between the peptides themselves are
probably due to convergence.
软体动物心脏兴奋性多肽FMRFamide(Phe-Met-
Arg-Phe-NH2)与四个结构相似的多肽一起,
构成了一个密切相关的核心家庭,现在包括
在其成员中,有两个四肽和三个七肽。
四肽和七肽具有不同的谱系分布
在软体动物中,具有独特的作用和受体
肌肉和神经。FMRFamide的抗血清也会发生交叉反应
广泛存在于神经和内分泌细胞中的非
软体动物类群,包括脊椎动物和无脊椎动物。这其中的一些
免疫反应是由已建立的多肽引起的,但之前
未知的多肽也已被发现。这些
免疫化学结果是一种大的,
异质、广泛、扩展的多肽家族;它是
其特征是具有以下形式的酰胺化C-末端二肽
Arg-X-NH2(式中X为芳香族残渣)。第一个长期的
该项目的目标是检查组织的分布、作用
以及FMRFamide相关个体的生理作用
组成模式核心家族的多肽(FARP)
肺蜗牛,螺旋藻。与之互补的受体
FARP的特征将通过研究结构-
这些多肽在整个组织上的活性关系(SAR),
生化机制与放射性配基-受体结合
属性。FARP的水平和效力的变化,
作为蜗牛活动状态的函数,也将是
检查过了。第二个长期目标是确定
FARP扩展家族的分布、大小和限制,以及
以确定其与软体动物核心家族的关系。
中枢神经将寻找新的软体外FARP
脊椎动物和关键无脊椎动物的提取物。的影响,
脊椎动物血管上的核FARP和延伸FARP
将对平滑肌进行测试,受体的特征是
萨尔。FARP核心家族与FARP的关系
大家庭可能存在于它们的同源中
受体;这些多肽之间的相似性是
可能是因为汇聚。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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MICHAEL J GREENBERG其他文献
MICHAEL J GREENBERG的其他文献
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{{ truncateString('MICHAEL J GREENBERG', 18)}}的其他基金
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