ADENOSINE AND CATECHOLAMINE INTERACTION IN THE HEART

腺苷和儿茶酚胺在心脏中的相互作用

• 批准号: 3485795
• 负责人: JAMES G DOBSON
• 金额: $ 19.8万
• 依托单位: UNIV OF MASSACHUSETTS MED SCH WORCESTER
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-07-01 至 1997-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3485795
• 关键词: adenosine; adenosinetriphosphatase; adenylate cyclase; beta adrenergic agent; calcium; catecholamines; cellular pathology; coronary disorder; cyclic AMP; fluorescent dye /probe; guinea pigs; heart circulation; heart contraction; heart failure; hormone regulation /control mechanism; isolation perfusion; laboratory rat; membrane transport proteins; oxygen consumption

The long-term objective of this project is to investigate the hypothesis that adenosine functions as a negative-feedback regulator of catecholamine-elicited responses in the mammalian heart. One specific aim is to reveal the importance of extracellular (interstitial) adenosine in the antiadrenergic actions of the nucleoside in isolated working hearts. Another specific aim is to investigate the ability of adenosine to modulate intracellular calcium of adult primary cultured ventricular myocytes using an intracellular calcium sensitive fluorescent indicator. Finally, and of primary importance are the studies designed to investigate the membrane biochemical mechanisms by which adenosine, upon interaction with its inhibitory receptor, manifests its antiadrenergic actions in the myocardial cell. For the last series of studies cultured ventricular myocytes and ventricular membranes will be utilized. The knowledge gained from this project should contribute significantly to the physiological characterization of the antiadrenergic action of adenosine in the normal working heart. The new knowledge should also provide insight into the way in which mechanisms regulating myocardial contractile state are altered in the failing and hypoxic heart, such as in conditions of cardiac and coronary artery disease.

该项目的长期目标是调查 腺苷作为负反馈的假设 哺乳动物中的儿茶酚胺引起的反应的调节剂 心。 一个具体的目的是揭示 抗氧化作用中的细胞外（间质）腺苷 在孤立的工作心脏中的核苷。 另一个具体目标 是研究腺苷调节细胞内的能力 成人原发性培养的心室肌细胞的钙使用 细胞内钙敏感荧光指示剂。 最后，然后 最重要的是旨在研究的研究 腺苷的膜生化机制 与其抑制受体的相互作用，表现 心肌细胞中的抗氧化作用。 上一个系列 研究培养的心室肌细胞和心室 膜将被利用。 从中获得的知识 项目应为生理学做出重大贡献 腺苷在 正常工作的心脏。 新知识也应提供 深入了解调节心肌的机制 收缩状态在失败和低氧心脏中发生了变化，这样 如心脏和冠状动脉疾病的状况。