SOLID PHASE CAPTURE FOR PCR SAMPLE PREPARATION

用于 PCR 样品制备的固相捕获

• 批准号: 2069303
• 负责人: FRANK A WHITE
• 金额: $ 4.6万
• 依托单位: SRA TECHNOLOGIES, INC.
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-09-01 至 1995-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2069303
• 关键词: DNA; RNA; human immunodeficiency virus 1; human subject; method development; nucleic acid hybridization; nucleic acid probes; nucleic acid sequence; physical separation; polymerase chain reaction; synthetic nucleic acid; virus RNA

The simplification of methods for the analysis of amplified nucleic acid sequences has largely focused on the development of nonradioisotopic detection reagents, and on the development of instrument systems to perform PCR in microwell plates. Sample preparation methods remain largely unchanged from techniques described a decade or more ago. While DNA extraction is relatively simple, most methods have concentrated on single tube techniques, which remain labor-intensive and whose application in screening tests is still problematic. The labile nature of RNA extracts makes reproducible, robust RNA extraction for analysis even more difficult. SRA Technologies, Inc. (SRA) proposes to apply affinity based hybrid collection techniques developed in house for PCR product detection and quantitation, to the problem of sample preparation for PCR in a screening assay. We further propose to develop these methods for specific application to the quantitation of HIV-1 titers in genital secretions, a problem of immediate relevance in the study of HIV- 1 sexual transmission.

扩增核酸分析方法的简化