ISOLATION OF PROTEOLYTIC ANTIBODIES IN E COLI
ISOLATION OF PROTEOLYTIC ANTIBODIES IN E COLI
批准号:
3489647
负责人:
Alan Greener
金额:
$4.93万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1993
资助国家:
美国
项目状态:
已结题
起止时间:
1993-01-15 至 1993-07-14
关键词:
Escherichia coli antibody antigen antibody reaction antigens chimeric proteins endopeptidases fusion gene genetic library laboratory mouse method development molecular cloning molecular genetics oligonucleotides polymerase chain reaction proteolysis radiotracer transfection /expression vector western blottings
中文摘要
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英文摘要
The objective of this proposal is to isolate antibody molecules capable
of proteolytic cleavage of their cognate antigen. An E. coli selection
system has been devised to identify these antibodies. Large antibody
libraries can be made and screened in E. coli using the ImmunoZapTM
technology, and this directed approach to the isolation of rare
antibodies with proteolytic activity may lead to new types of therapeutic
agents for combating certain infections and diseases.
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Developing an Improved Electrocompetent E. coli Host
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批准号:8590717
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资助金额:$14.33万
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财政年份:2013
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批准号:8053538
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资助金额:$11.37万
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财政年份:2010
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Genetic Method for Evaluating Solubility of Recombinant Proteins in E. coli
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批准号:7272222
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项目类别:
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资助金额:$11.48万
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财政年份:2007
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负责人:Alan Greener
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依托单位:
Screening Complete TB Proteome for Protective Antigens
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批准号:7287837
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项目类别:
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资助金额:$80.39万
-
财政年份:2006
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负责人:Alan Greener
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依托单位:
Screening Complete TB Proteome for Protective Antigens
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批准号:7493092
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项目类别:
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资助金额:$87.93万
-
财政年份:2006
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负责人:Alan Greener
-
依托单位:
Screening Complete TB Proteome for Protective Antigens
-
批准号:7053631
-
项目类别:
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资助金额:$82.2万
-
财政年份:2006
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负责人:Alan Greener
-
依托单位:
GENETIC METHOD TO ANALYZE BACS AND CONSTRUCT CONTIG MAPS
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批准号:2828661
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项目类别:
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资助金额:$9.96万
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财政年份:1999
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负责人:Alan Greener
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依托单位:
INFECTION OF MAMMALIAN CELLS WITH BACTERIOPHAGE LAMBDA
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批准号:2540377
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项目类别:
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资助金额:$9.91万
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财政年份:1997
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负责人:Alan Greener
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依托单位:
OPTIMIZATION OF PROTEOLYTIC CLEAVAGE OF PEPTIDE AFFINITY
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批准号:2192656
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项目类别:
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资助金额:$4.87万
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财政年份:1995
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负责人:Alan Greener
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依托单位:
ISOLATION OF INTERACTIVE PROTEINS IN E COLI
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批准号:2253895
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项目类别:
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资助金额:$6.41万
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财政年份:1994
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负责人:Alan Greener
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依托单位:
EXTRACELLULAR SECRETION OF PROTEINS IN E COLI
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批准号:2071248
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项目类别:
-
资助金额:$7.22万
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财政年份:1994
-
负责人:Alan Greener
-
依托单位:
NEW BACTERIAL HOSTS FOR EXPRESSING FOREIGN PROTEINS
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批准号:2066692
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项目类别:
-
资助金额:$12.67万
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财政年份:1993
-
负责人:Alan Greener
-
依托单位:
NEW BACTERIAL HOSTS FOR EXPRESSING FOREIGN GENES
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批准号:3489531
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项目类别:
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资助金额:$4.29万
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财政年份:1991
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负责人:Alan Greener
-
依托单位:
海外基金