IMMOBILIZED OXIDO-REDUCTASE ENZYMES IN ORGANIC SYNTHESIS

需要新的试剂来生产光学纯的 制药公司。为此，我们建议评估和制定一项 用于有机合成的新型两相生物催化剂体系。这位将军 目标是开发一种依赖辅因子的方法学。 酶可用于在有机溶剂中进行立体定向 生产高价值的化合物，如药品。具体的 第一阶段的目标是证明马肝酒精 脱氢酶及其辅因子烟酰胺腺嘌呤 二核苷酸(NADH)，可以固定在水凝胶中并在 从光学合成光学纯化合物的二乙醚 不活跃的前驱物质。第二阶段将开发这种新的合成材料 低成本生产的一般程序方法论 依赖辅因子的药物和农业化合物 有机溶剂中的氧化还原酶。这三位研究人员 提交此建议书已成功合作开发了 固定化、不依赖辅因子的立体选择性酶系统 有机溶剂中的水解和酯交换反应。

There is a need for new reagents for the production of optically pure pharmaceuticals. For this purpose, we propose to evaluate and develop a new biphasic biocatalyst system for organic synthesis. The general objective is to develop a methodology in which cofactor dependent enzymes can be used in organic solvents for the stereospecific production of high value compounds such as pharmaceuticals. The specific Phase I objective is to demonstrate that horse liver alcohol dehydrogenase (HLADH), along with its cofactor nicotinamide adenine dinucleotide (NADH), can be immobilized in a hydrogel and operate in diethyl ether to produce optically pure compounds from optically inactive precursors. Phase II will develop this new synthetic methodology into general procedures for the low cost production of pharmaceutical and agricultural compounds using cofactor dependent oxido-reductase enzymes in organic solvents. The three researchers submitting this proposal have collaborated successfully to develop an immobilized, cofactor independent, enzyme system for stereoselective hydrolysis and transesterification in organic solvents.