ANTIBODIES TO HUMAN PANCREATIC TRYPSIN AND TRYPSINOGEN

这项建议涉及制定程序，以 人胰酶和发酵原的纯化及对 多克隆抗体和单抗的研制。 具体地说，这项提案的目的是让人类 阳离子胰酶原，阳离子胰酶，凝乳酶原I， 胰凝乳酶1、脂肪酶和淀粉酶，并将其用作来源 用于生产多克隆和单克隆的抗原 抗体。然后，这些抗体将在第二阶段用于 建立其在血清和其他组织中的检测方法 体液。胰腺蛋白，它们的多克隆和 单抗和酶联免疫吸附试验应可用于 胰腺检测试剂盒的商业化开发 通过检测血清中的胰腺蛋白发现异常。

This proposal involves the development of procedures for the purification of human pancreatic enzymes and zymogens and for the development of polyclonal and monoclonal antibodies. Specifically, the aims of this proposal are to prepare human cationic trypsinogen, cationic trypsin, chymotrypsinogen I, chymotrypsin 1, lipase, and amylase, and to use these as sources of antigens for the production of both polyclonal and monoclonal antibodies. These antibodies will then be utilized in Phase II to develop ELISA assays for their measurement in serum and other fluids. Both the pancreatic proteins, their polyclonal and monoclonal antibodies, and the ELISA assays should be useful for the commercial development of assay kits to measure pancreatic abnormalities though the detection of pancreatic proteins in serum.