DNA PROBE IN DIAGNOSIS OF CAMPYLOBACTER PYLORI GASTRITIS

一种快速、准确、易用、低成本的DNA探针检测试剂盒 相关微生物幽门弯曲菌的检测 患有胃炎和消化性溃疡的，将被开发用于 内窥镜检查诊所和病理实验室。使用全基因组 或短的非同位素形式的合成DNA探针，这种测试 将允许快速检测幽门螺杆菌总核酸或 分别在内窥镜检查中发现16S核糖体RNA 活检、胃粘液、胃液或粪便。这项测试将 包括固相DNA探针和标记DNA溶液相 探测器。将在以下时间检测到目标核酸 形成由固相组成的杂化络合物 DNA探针、靶核酸和标记的溶液相 DNA探针。商业DNA原型的最终配置 提出了探头检测试剂盒产品，并对该产品进行了评价 通过有限的临床试验测试试剂盒。

A rapid, accurate, easy-to-use and low-cost DNA probe test kit for the detection of Campylobacter pylori, the microorganism associated with gastritis and peptic ulcer, will be developed for use in the endoscopy clinic and the pathology laboratory. Using whole genomic or short synthetic DNA probes in a non-isotopic format, this test will allow the rapid detection of C. pylori total nucleic acid or 16S ribosomal RNA, respectively, when present in endoscopic biopsies, stomach mucous, gastric juice or feces. The test will include a solid phase DNA probe and a solution phase labeled DNA probe. The target nucleic acid will be detected following formation of a hybridization complex consisting of the solid phase DNA probe, the target nucleic acid, and the solution phase labeled DNA probe. A final configuration of the prototype commercial DNA probe test kit product is proposed, as well as an evaluation of the test kit through a limited clinical trial.