INTERPHASE CYTOGENETICS: CHROMOSOME-SPECIFIC DNA PROBES

染色体异常检测的最新技术 需要细胞培养和制备 分裂中期染色体 间期细胞遗传学使用原位 荧光标记的DNA探针的杂交是潜在的 快速，相对便宜，并适合自动化。 是 这项研究的目的是产生染色体特异性DNA， 适用于检测染色体异常的探针， 数字变异、易位和缺失。 具体 该建议的目的是（1）选择克隆的子集， 从染色体7 HTF岛定位到不同的区域， 染色体7和（2）以评价这些克隆的能力， 装饰中期和间期染色体。

Current techniques for the detection of chromosome anomalies are time consuming and require cell culture and the preparation of metaphase chromosomes. Interphase cytogenetics using in situ hybridization of fluorescent labeled DNA probes is potentially rapid, relatively inexpensive, and lends itself to automation. It is the goal of this research to generate chromosome-specific DNA probes suitable for detection of chromosomal anomalies including numerical variations, translocations and deletions. The specific aims of this proposal are (1) to select a subset of clones derived from chromosome 7 HTF islands localized to various regions of chromosome 7 and (2) to evaluate these clones for the ability to decorate metaphase and interphase chromosomes.