HIGH-RESOLUTION MUTAGENESIS

探讨了高分辨率诱变在 L5178 Y小鼠淋巴瘤细胞中的胸苷激酶（TK）基因座将 进行检查（alpha）以更彻底地评估假设 在L5178 Y的双峰分布中， 细胞突变是由于更广泛的损害， TK基因区域（染色体11b）和大区域（λ） 集落由TK基因的点突变产生，和（B）由TK基因的点突变产生， 表征L5178 Y小鼠中产生的omega和lambda突变体 菌株3.7.2C、LY-S1和LY-R83在叶酸缺乏的 然后将培养基暴露于乙基甲烷磺酸盐。 表型 TK和半乳糖激酶（GK）基因座的突变培养物将 特征;高分辨率带染色体（11号） 将进行分析，并在原位杂交的放射性探针， TK和GK cDNA的高分辨率显带染色体， 被启动。 在第二阶段，将继续采用这些办法， 将在分子水平上表征突变体。

The feasibility of probing high resolution mutagenesis at the thymidine kinase (TK) locus in L5178Y mouse lymphoma cells will be examined (alpha) to more thoroughly evaluate the hypothesis that small sigma colonies in the bimodal distribution of L5178Y cells mutants are the result of more extensive damage to the region of the TK genes (chromosome 11b) and that large (lambda) colonies result from point mutations to the TK gene, and (b) to characterize omega and lambda mutants arising in L5178Y mouse strains 3.7.2C, LY-S1, and LY-R83 grown in folic acid-deficient medium then exposed to ethylmethane sulfonate. The phenotype of mutant cultures at the TK and galactokinase (GK) loci will be characterized; high resolution banded chromosomes (number 11) will be analyzed, and in situ hybridization of radioactive probes of TK and GK cDNA to the high-resolution banded chromosomes will be initiated. In Phase II these approaches will be continued and the mutants will be characterized at the molecular level.