SERUM-FREE MEDIUM FOR LAK, NK CELLS AND MONOCYTES

这项拟议研究的长期目标是开发血清- 用于培养人白细胞的自由介质配方 体外培养。第一阶段研究的目标是发展无血清 能够最大限度地生长和发挥细胞毒活性的培养基 非丝裂原激活的人淋巴因子激活的杀伤细胞， 自然杀伤细胞、单核细胞和丝裂原激活的细胞毒T细胞 细胞。根据第一阶段的结果，第二阶段的研究将集中 用于肿瘤批量生产的无血清培养基料的研制 用于医学研究的患者来源的白细胞。这种媒介会 缓解与细胞繁殖相关的许多问题 动物血清或混合的人血清的存在。此阶段I 提案概述了高质量无血清的发展 利用完全来自人类的生物成分的介质 消息来源。每种药物的组成和最佳浓度 在选择介质的组件时要特别注意 用于人类细胞的生长和维持。通过控制 外部信号在各种生物反应调节剂中的作用 对细胞毒活性的发挥可以清楚地进行评价。

The long range goal of the proposed research is to develop serum- free media formulations for the culture of human leukocytes in vitro. The objective of Phase I studies is to develop serum-free medium that would allow maximum growth and cytotoxic activity of non-mitogen activated human lymphokine activated killer cells, natural killer cells, monocytes and mitogen activated cytotoxic T cells. Based on Phase I results, Phase II studies will concentrate on producing serum-free medium for the batch production of cancer patient-derived leukocytes for medical research. This medium would alleviate many of the problems associated with cell propagation in the presence of animal sera or pooled human sera. This Phase I proposal outlines the development of a high quality serum-free medium utilizing biological components derived solely from human sources. The composition and optimal concentrations of each component of the medium will be selected with particular concern for the growth and maintenance of human cells. By controlling the external signals the role various biological response modifiers play on cytotoxic activity can be clearly evaluated.