DETECTION OF HYBRIDIZATION WITHOUT RADIOACTIVE LABEL

无放射性标记的杂交检测

• 批准号: 3493577
• 负责人: QIANG LI
• 金额: $ 5万
• 依托单位: ADELPHI TECHNOLOGY, INC.
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-08-04 至 1994-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3493577
• 关键词: biomedical equipment development; nucleic acid hybridization; surface property

A novel surface phenomenon will be explored for the quantitative detection of nucleic acid hybridization. We will construct a relatively inexpensive instrument, which can attain a sensitivity limit comparable to the existing methodologies, without the use of dyes, or radioisotopes. As part of the phase l research, we will use the existing hardware to test affinity of DNA to a specifically prepared surface. This test is a necessary step in the operation of the instrument. We will also measure the binding of the complementary DNA to assess the detection limit, sensitivity and the issue of non-specific binding. In Phase Il, the signal to noise ratio will be optimized and a prototype of the device specifically designed for hybridization detection will be built. The complete hybridization protocol will be tested on the new instrument. If the proposed research is successful, the new instrument can perform routine nucleic acid hybridization detection for molecular biology research and clinical laboratory tests.

一种新的表面现象将被探索的定量检测 核酸杂交。我们将建造一个相对便宜的 仪器，它可以达到一个灵敏度限制可比的 现有的方法，而不使用染料或放射性同位素。一部分 在第一阶段的研究中，我们将使用现有的硬件进行测试， DNA对特定表面的亲和力。这个测试是一个 这是仪器操作中的一个必要步骤。我们还将测量 互补DNA的结合以评估检测限， 敏感性和非特异性结合的问题。在阶段II中，信号 将优化信噪比，并制作出该设备的原型 将建立专门为杂交检测而设计的。的 将在新仪器上测试完整的杂交方案。如果 建议的研究是成功的，新仪器可以执行 分子生物学常规核酸杂交检测 研究和临床实验室测试。