DETECTION OF HYBRIDIZATION WITHOUT RADIOACTIVE LABEL

一种新的表面现象将被用于定量检测。 核酸杂交。我们将建造一个相对便宜的 仪器，它可以达到与 现有的方法，没有使用染料或放射性同位素。作为一部分 对L阶段的研究，我们将利用现有的硬件进行测试 DNA对特殊准备的表面的亲和力。这项测试是一项 仪器操作中的必要步骤。我们还将衡量 互补DNA的结合以评估检测下限， 敏感性和非特定约束性问题。在阶段Il中，信号 信噪比将得到优化，并制作出该设备的原型 将建造专门用于杂交检测的设备。这个 完整的杂交方案将在新仪器上进行测试。如果 所提出的研究是成功的，新的仪器可以执行 分子生物学中的常规核酸杂交检测 研究和临床实验室测试。

A novel surface phenomenon will be explored for the quantitative detection of nucleic acid hybridization. We will construct a relatively inexpensive instrument, which can attain a sensitivity limit comparable to the existing methodologies, without the use of dyes, or radioisotopes. As part of the phase l research, we will use the existing hardware to test affinity of DNA to a specifically prepared surface. This test is a necessary step in the operation of the instrument. We will also measure the binding of the complementary DNA to assess the detection limit, sensitivity and the issue of non-specific binding. In Phase Il, the signal to noise ratio will be optimized and a prototype of the device specifically designed for hybridization detection will be built. The complete hybridization protocol will be tested on the new instrument. If the proposed research is successful, the new instrument can perform routine nucleic acid hybridization detection for molecular biology research and clinical laboratory tests.