CHOLESTEROL THRESHOLD ASSAY

胆固醇阈值测定

• 批准号: 3501504
• 负责人: Mary Gaunt-Kloepfer
• 金额: $ 5万
• 依托单位: MICRONIX, INC.
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-08-01 至 1990-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3501504
• 关键词: blood chemistry; cholesterol; diagnosis design /evaluation; hypercholesterolemia; mass screening; metabolism disorder diagnosis

Proposed is the development of a blood test strip for cholesterol. Plasma is separated on the strip from cells by a patented filtration process through a glass fiber fleece, collecting the plasma on a detachable recipient member, and eluting it into a liquid reagent. Cholesterol esterase (CHE) and oxidase (CHOD) are incorporated in dry form on the strip. Quantitation is based on a "threshold" assay for peroxide, generated by CGE and CHOD. The assay comprises a "scavenger" and a "signal" reaction. The below threshold amount of peroxide, equivalent to the 200 mg/dL cholesterol cutoff recently suggested by the NHLBI, is reacted in the scavenger, the above threshold amount in the signal reaction. Thus, by elimination of large color backgrounds, and choosing highly sensitive signal reactions, visual interpretation is greatly enhanced. The device does not require an instrument making it economical and fast for mass screenings and self-monitoring. Feasibility of the test principle has been demonstrated for peroxide and cholesterol from serum. The proposed work comprises final selection of optimal plasma separation and recipient member materials, optimization of the reagent system, and the manufacture of functional prototype test strips capable of differentiating concentration intervals of between 10 and 20 mg/dL around the 200 mg/dL cutoff level.

提议开发胆固醇血液测试条。 通过专利过滤将血浆与细胞分离 通过玻璃纤维绒进行处理，将血浆收集在 可拆卸的接受部件，并将其洗脱到液体试剂中。 胆固醇酯酶 (CHE) 和氧化酶 (CHOD) 被纳入干 形成在条带上。 定量基于“阈值”测定 过氧化物，由 CGE 和 CHOD 产生。该测定包含“清除剂” 和“信号”反应。 过氧化物含量低于阈值， 相当于最近建议的 200 mg/dL 胆固醇临界值 NHLBI，在清除剂中发生反应，上述阈值量在 信号反应。 因此，通过消除大的彩色背景，和 选择高度敏感的信号反应，视觉解释是 大大增强。 该设备不需要仪器来制造它 经济、快速地进行大规模筛查和自我监测。 可行性 过氧化物和胆固醇的测试原理已得到证实 来自血清。 拟议的工作包括最佳血浆分离的最终选择 和接收成员材料，试剂系统的优化，以及 功能原型测试条的制造能够 区分 10 至 20 mg/dL 之间的浓度区间 200 mg/dL 截止水平。