MINIATURE DIAGNOSTIC DEVICE FOR SERUM CHOLESTEROL

这项研究的总体目标是开发一种微型， 一种独立于机器的等离子体定量诊断装置 胆固醇，适用于人体内胆固醇的测定。 医生办公室的家。建议进行的具体研究 第一阶段涉及结合以前开发的一种胆固醇- 具有胆固醇的专用模拟-数字化学颜色开关 酯酶活性，以建立起作用于 脂蛋白胆固醇酯和游离胆固醇 脂蛋白颗粒。系统的优化使用 还将采用序贯单纯形法。第一阶段是 旨在证明建立胆固醇的可行性 基于a/d颜色开关的诊断设备。后续 阶段将湿化学还原为干形式，并产生 原型设备。

The overall object of this research is to develop a miniature, machine-independent, quantitative diagnostic device for plasma cholesterol, that is suitable for cholesterol determination in the home of the doctor's office. The specific research proposed for Phase 1 deals with combining a previously developed cholesterol- specific analog-to-digital chemical color switch with cholesterol esterase activity, to build a reaction matrix that will act on lipoprotein cholesterol esters as well as free cholesterol in lipoprotein particles. Optimization of the system using the sequential simplex method will also be undertaken. Phase I is intended to demonstrate the feasibility of building the cholesterol diagnostic device, based on the a/d color switch. Subsequent phases will reduce the wet chemistry to a dry format, and produce prototype devices.