ISOLATION OF BULK DEOXYNUCLEOSIDES FOR AIDS DRUGS
ISOLATION OF BULK DEOXYNUCLEOSIDES FOR AIDS DRUGS
批准号:
3506042
负责人:
JOSEPH A TOCE
金额:
$36.83万
依托单位国家:
美国
项目类别:
财政年份:
1988
资助国家:
美国
项目状态:
已结题
起止时间:
1988-04-01 至 1990-04-30
中文摘要
脱氧核苷目前在
英文摘要
Deoxynucleosides are currently playing an increasing role in
medicine. Several experimental anti-viral AIDS drugs (AZT, DCC,
AND DDA) are chemically modified deoxynucleosides. These drugs are
expensive due to the unavailability of deoxynucleosides at
reasonable costs. Deoxynucleosides are also in demand as nutrients
in mammalian tissue cultures used to produce monoclonal antibodies
and other medically significant blood products. A minor modified
component of eukaryotic DNA is 5-methyldeoxycytosine. It is needed
for the study of gene expression control and for chemical synthesis
of modified DNA fragments used in recombinant DNA technology.
Considering the need for anti-viral drugs and cloned blood products
and the importance of time and cost, and Phase II research program
is proposed for the immediate availability of specific
deoxynucleosides. The proposed research consists of four specific
segments: a) optimization of the enzymatic hydrolysis reaction
while reducing the level of deamination in the deoxynucleoside
mixture, b) scaling up the ion-exlusion column and developing the
secondary chromatographic column required for final purification
of each deoxynucleoside, c) finalize procedures to product
crystalline deoxynucleosides and d) establish quality control
specifications and explore methods for purifying 5-
methyldeoxycytosine. Cost analysis indicates that optimization of
the Phase I innovations will result in a significant price
reduction of deoxynucleosides.
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SYNTHESIS OF OXIDANT-FREE POTASSIUM LACTOBIONATE
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批准号:3489760
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项目类别:
-
资助金额:$5.0万
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财政年份:1993
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负责人:JOSEPH A TOCE
-
依托单位:
SYNTHESIS AND TESTING OF A NEW CLASS OF HEPARINOIDS
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批准号:3502068
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项目类别:
-
资助金额:$5.0万
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财政年份:1991
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负责人:JOSEPH A TOCE
-
依托单位:
SYNTHESIS AND TESTING OF A NEW CLASS OF HEPARINOIDS
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批准号:2750359
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项目类别:
-
资助金额:$26.76万
-
财政年份:1991
-
负责人:JOSEPH A TOCE
-
依托单位:
SYNTHESIS AND TESTING OF A NEW CLASS OF HEPARINOIDS
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批准号:2028650
-
项目类别:
-
资助金额:$31.98万
-
财政年份:1991
-
负责人:JOSEPH A TOCE
-
依托单位:
SYNTHESIS AND TESTING OF A NEW CLASS OF HEPARINOIDS
-
批准号:6349584
-
项目类别:
-
资助金额:$10.99万
-
财政年份:1991
-
负责人:JOSEPH A TOCE
-
依托单位:
ISOLATION OF BULK DEOXYNUCLEOSIDES FOR AIDS DRUGS
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批准号:3488793
-
项目类别:
-
资助金额:$5.0万
-
财政年份:1988
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负责人:JOSEPH A TOCE
-
依托单位:
海外基金