FILM SCANNER FOR HIGH THROUGHPUT DNA SEQUENCING

这项研究的总体目标是开发一种高度自动化的DNA 可以卖给分子生物学研究人员的测序系统 有兴趣进行大规模测序项目。 完整的 该系统将包括机器人反应制备、高容量凝胶 电泳系统，机器人凝胶加载，自动凝胶膜读取器和 管理数据的软件。 本提案的具体目标仅限于发展一个 组件，自动放射自显影胶片阅读器。 我们的目标是一个凝胶阅读器的能力，从10,000至20000 在大约一小时内，每个X光片的原始序列的基础， 用户的注意力，并将能够制造 以低于3万美元的价格投放市场。

The overall objective of this research is to develop a highly automated DNA sequencing system that can be sold to molecular biology researchers interested in carrying out large scale sequencing projects. The complete system will include robotic reaction preparation, high capacity gel electrophoresis system, robotic gel loading, automatic gel film reader and software for managing the data. The specific aim of this proposal is limited to development of one component, the automatic autoradiographic film reader. We aim for a gel reader with the capability to read from 10,000 to 20,000 bases of raw sequence per x-ray film in about one hour with a minimum of attention from the user and which will be able to be manufactured inexpensively enough to be placed on the market at less than $30,000.