NOVEL IMMOBILIZED ENZYME ELECTRODE

新型固定化酶电极

• 批准号: 3495986
• 负责人: E JENNINGS TAYLOR
• 金额: $ 5万
• 依托单位: PHYSICAL SCIENCES, INC
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-05-01 至 1990-10-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3495986
• 关键词: biosensor device; clinical biomedical equipment; electrochemistry; enzyme electrodes

The proposed R&D is directed towards the development of improved immobilized enzyme biosensor technology. Immobilized enzyme biosensors are important components of; 1) in-vivo monitoring; and 2) clinical analysis systems. to date, the major problems with these biosensors are instability due to poor enzyme immobilization techniques and limited sensitivity to amperometric detection of peroxide, the reaction product detected by the sensor. In Phase I, we propose to demonstrate two separate innovations addressing these two problems; 1) a novel enzyme immobilization procedure, and 2) a novel method for quantitative peroxide detection. Taken together, these innovations have the potential to significantly impact biosensor technology for a variety of application. Our Phase I efforts are directed towards the development of a Glucose Oxidase biosensor for glucose detection incorporating our innovations. However, our innovations are widely applicable to other immobilized enzyme and immobilized enzyme- mediator biosensors.

建议的研发是针对发展改进的 固定化酶生物传感器技术 固定化酶生物传感器 重要组成部分; 1）体内监测;和2）临床分析 系统. 迄今为止，这些生物传感器的主要问题是不稳定性 由于差的酶固定化技术和有限的灵敏度， 过氧化氢的安培检测，反应产物由 传感器. 在第一阶段，我们建议展示两个独立的创新 解决这两个问题：1）新的酶固定化方法， 和2）定量过氧化物检测的新方法。 综合起来看， 这些创新具有显著影响生物传感器的潜力 用于各种应用技术。 我们的第一阶段工作是针对 葡萄糖氧化酶生物传感器的发展 检测结合我们的创新。 然而，我们的创新是 广泛适用于其它固定化酶及固定化酶- 介体生物传感器