CLONING OF THE SEROTONIN 5-HT 1P RECEPTOR

5-羟色胺5-HT受体已被广泛表征， 肠神经系统，其功能是调节 胃肠蠕动 此外，5-HT样结合位点已被发现。 在心包膜和心内膜中发现， 皮肤和脑血管的部分， 受体可能在偏头痛中起作用。 这些网站没有被广泛使用。 由于难以解剖相对较小的数量， 神经组织和非神经组织的区别 5-HT受体的分子克隆和异源表达将为进一步研究5-HT受体的功能奠定基础。 极大地促进了这种受体的研究，并将提供一个关键的工具， 发现影响该受体的药物。 两 电生理和受体结合数据表明，5-HT 受体是G蛋白受体超家族的成员。 但 这种受体所连接的第二信使从未被 测定 阐明第二信使途径在第一阶段的这一点 该项目将使我们能够设计一个克隆策略。 第二阶段a 基于PCR和同源性筛选技术的克隆策略将是 将克隆的受体转染到哺乳动物细胞中 用于高通量药物发现系统的生产线。

The serotonin 5-HT receptor has been extensively characterized in the enteric nervous system where it functions in the regulation of gastrointestinal motility. In addition, 5-HT like binding sites have been discovered in the pericardium and endocardium of the heart, the innervation of the skin, and on portions of the cerebral vasculature where these receptors might play a role in migraine. These sites have not been widely studied due to the difficulty in dissecting the relatively small quantities of neural tissue from the non-neural tissue in these locations. Molecular cloning and heterologous expression of the 5-HT receptor will greatly facilitate study of this receptor and will provide a key tool for the discovery of drugs affecting this receptor site. Both electrophysiological and receptor binding data indicate that the 5-HT receptor is am member of the G protein receptor superfamily. However, the second messenger to which this receptor is coupled has never been determined. Elucidation of the second messenger pathway in phase I of this project will allow us to design of a cloning strategy. In phase II, a cloning strategy based on PCR and homology screening techniques will be implemented and the cloned receptor will be transfected into mammalian cell lines for use as high-throughput drug discovery system.