ULTRA-THIN DIAMOND WIRE SAW FOR HISTOLOGY

该项目的具体目标是制造、开发和评估 一种用于生物切片的超薄人造金刚石绳锯 样本。直径6-12(亩)米的钼、钨或不锈钢 采用等离子体辅助化学气相沉积技术对金属丝进行金刚石涂层 (PACVD)以产生磨料涂层。亚微米级钻石 谷物将以受控的方式定期沉积在 那根电线。现有的绳锯机将被用来切割各种 组织样本。目标是实现1(亩)米-50(亩)米厚的截面，用于 新鲜组织、难切割骨、牙齿的光镜评价 或软骨组织，各种硬度和大直径的组织 高质量的生物体样本器官 技巧。这项技术在医疗应用中的用处 在免疫细胞化学、酶组织化学、诊断等领域 活组织标本，药物的效果是可以预期的。从长远来看 目标是将一种组织学绳锯机商业化 现有缩微胶卷的替代技术或辅助技术。这台机器 将在许多方面克服传统缩微型书的局限性 应用，并将使更精细和更多分子成为可能 对各种组织进行分析。

The specific aim of this project is to manufacture, develop and evaluate an ultra-thin synthetic diamond wire saw for sectioning biological specimen. 6-12 (mu)m in diameter molybdenum, tungsten or stainless steel wires will be diamond coated by Plasma Aided Chemical Vapor Deposition (PACVD) to create the abrasive coating. The submicron sized diamond grains will be deposited in a controlled fashion at regular intervals on the wire. An existing wire saw machine will be used to section various tissue samples. The goal is to achieve 1(mu)m-50(mu)m thick sections for light microscopic evaluation of fresh tissue, hard to section bone, tooth or cartilage tissue, tissue of various hardnesses and large diameter organ of organism samples of higher quality than is available by current techniques. The usefulness of the technology for medical applications in such fields as immunocytochemistry, enzyme histochemistry, diagnosis of biopsy specimens, and effects of drugs is anticipated. The long term goal is to commercialize a histological wire saw machine as an alternative or ancillary technology to existing microtomes. This machine will overcome the limitations of conventional microtomes in a number of applications and will also make possible a finer and more molecular analysis of various tissues.