MECHANISMS - MICRONUCLEI INDUCTION IN MARROW

描述：（主要研究者摘要）由于速度和 微核（MN）鉴定简单，MN分析具有 越来越多地被用作一种敏感的，但成本较低， 传统中期分析的替代品。 MN评分 是目前可用于检测的少数方法之一 非整倍体相关过程。 尽管已经进行了几次MN测定， 开发的，最广泛使用的测试系统涉及MN的评分， 小鼠骨髓或外周血嗜多染红细胞（PCE）。 事实上，MN也可以通过一种不依赖于DNA的机制产生。 损伤既是一个重要属性，也是一个混淆因素。 因此 发展和应用技术，以确定机制， 在MN数据的解释之前， 变得可靠。 抗染色体抗体的发现 动粒区使得我们有可能确定 的MN，该方法已在体外用于鉴定MN， 细胞质分裂异常 该项目的目的是开发和 将该技术应用于由遗传毒性剂在小鼠中诱导的MN。

DESCRIPTION: (Principal Investigator's Abstract) Due to the speed and simplicity involved in micronuclei (MN) identification, MN analysis has become increasingly used as a sensitive, yet less expensive and time consuming alternative to classical metaphase analysis. The scoring of MN is one of the few methods currently available for the detection of aneuploid-related processes. Although several MN assays have been developed, the most widely used test system involves the scoring of MN in bone marrow or peripheral blood polychromatic erythrocytes (PCE) of mice. The fact that MN can also arise through a mechanism independent of DNA damage is both an important attribute and a confounding factor. Thus, the development and application of techniques for identifying the mechanistic origin of MN are required before the interpretation of MN data can ever become reliable. The discovery of antibodies against chromosome kinetochore regions has made it possible to identify the mechanistic origin of MN and this method has been used in vitro to identify MN which arise from abnormal cytokinesis. The purpose of this project is to develop and apply this technique to MN induced in mice by genotoxic agents.