MECHANISMS - MICRONUCLEI INDUCTION IN MARROW

机制 - 骨髓中的微核诱导

• 批准号: 3496626
• 负责人: ROLLAND R POUST
• 金额: $ 5万
• 依托单位: INTEGRATED LABORATORY SYSTEMS, LLC
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-07-01 至 1991-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3496626
• 关键词: DNA damage; antinuclear autoantibody; blood; bone marrow; cell cycle; centromere; chromosome aberrations; cytogenetics; erythrocytes; laboratory mouse; mutagen testing; stainings; technology /technique development

DESCRIPTION: (Principal Investigator's Abstract) Due to the speed and simplicity involved in micronuclei (MN) identification, MN analysis has become increasingly used as a sensitive, yet less expensive and time consuming alternative to classical metaphase analysis. The scoring of MN is one of the few methods currently available for the detection of aneuploid-related processes. Although several MN assays have been developed, the most widely used test system involves the scoring of MN in bone marrow or peripheral blood polychromatic erythrocytes (PCE) of mice. The fact that MN can also arise through a mechanism independent of DNA damage is both an important attribute and a confounding factor. Thus, the development and application of techniques for identifying the mechanistic origin of MN are required before the interpretation of MN data can ever become reliable. The discovery of antibodies against chromosome kinetochore regions has made it possible to identify the mechanistic origin of MN and this method has been used in vitro to identify MN which arise from abnormal cytokinesis. The purpose of this project is to develop and apply this technique to MN induced in mice by genotoxic agents.

描述：(首席调查员摘要)由于速度和 在微核(MN)鉴定中，MN分析具有简单性 越来越多地被用作敏感的、但不那么昂贵的时间 经典中期分析的消耗性替代。MN的得分 是目前为数不多的几种可用于检测 与非整倍体相关的过程。尽管已经有几种MN检测方法 开发的，最广泛使用的测试系统涉及MN在 小鼠骨髓或外周血多染红细胞(PCE)。 事实上，MN也可以通过独立于DNA的机制产生 损害既是一个重要因素，也是一个令人困惑的因素。因此， 机械设备识别技术的发展与应用 在解释MN数据之前，需要MN的来源 变得可靠。抗染色体抗体的发现 动粒区域使得识别机械起源成为可能。 该方法已在体外用于鉴定出现的MN 来自异常的细胞质分裂。该项目的目的是开发和开发 将这项技术应用于遗传毒性药物诱导的小鼠微核。