AUTOMATED PROTEIN SEQUENCING BY MICRO-LC/ELECTROSPRAY MS
通过 MICRO-LC/ELECTROSPRAY MS 进行自动蛋白质测序
基本信息
- 批准号:3498648
- 负责人:
- 金额:$ 5万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1991
- 资助国家:美国
- 起止时间:1991-04-01 至 1991-12-14
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The overall goal of this project is to develop a high-speed automated
protein sequencer employing high-performance packed capillary LC
separation, immobilized enzymes, and electrospray mass spectrometry. This
system should provide molecular weight and sequence information using
sample amounts in the 1-10 pmol level and complete sequences at the 10-100
pmol level. This new sequencer should be technically and economically
competitive with the modern gas phase Edman sequencer, but orders of
magnitude faster, and somewhat more reliable and versatile in that it
should be able to deal with blocked N-termini and unusual amino acid
residues. The proposed approach is based on earlier successful work using
standard-bore columns with thermospray mass spectrometry, but takes
advantage of the much higher sensitivities which are possible with packed
capillary LC interfaced to electrospray MS. An important new component to
the proposed approach is the combination of both aminopeptidases and
carboxypeptidases with collision induced dissociation of doubly charged
tryptic peptides to provide complete sequences on low picomole quantities
of tryptic fragments produced and isolated on-line. In phase I it will be
established that the proposed approach gives reliable, unambiguous sequence
information, and it will be shown that the proposed sensitivities are now
feasible within the present performance of commercial electrospray mass
spectrometers, further improvement in new mass spectrometric techniques
applicable to proteins and peptides are considered likely, and as the
sensitivity of the MS improves so will the sensitivity of this new approach
to sequencing.
该项目的总体目标是开发一种高速自动化
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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MARVIN L VESTAL其他文献
MARVIN L VESTAL的其他文献
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{{ truncateString('MARVIN L VESTAL', 18)}}的其他基金
Quantitative Measurement of Isotope Ratios by TOF-SIMS MS
通过 TOF-SIMS MS 定量测量同位素比
- 批准号:
8396848 - 财政年份:2012
- 资助金额:
$ 5万 - 项目类别:
Merged Beam MALDI TOF-TOF Using Ion-Ion Reactions to Determine Structure of
合并束 MALDI TOF-TOF 使用离子-离子反应确定结构
- 批准号:
7756913 - 财政年份:2010
- 资助金额:
$ 5万 - 项目类别:
Merged Beam MALDI TOF-TOF Using Ion-Ion Reactions to Determine Structure of
合并束 MALDI TOF-TOF 使用离子-离子反应确定结构
- 批准号:
8009479 - 财政年份:2010
- 资助金额:
$ 5万 - 项目类别:
Comprehensive Database System for Modern MALDI- TOF and TOF-TOF MS and MS-MS
现代 MALDI-TOF 和 TOF-TOF MS 和 MS-MS 的综合数据库系统
- 批准号:
7907124 - 财政年份:2010
- 资助金额:
$ 5万 - 项目类别:
Identification and Structural Elucidation of Proteins in MALDI-TOF-TOF MS-MS
MALDI-TOF-TOF MS-MS 中蛋白质的鉴定和结构解析
- 批准号:
8332393 - 财政年份:2010
- 资助金额:
$ 5万 - 项目类别:
Identification and Structural Elucidation of Proteins in MALDI-TOF-TOF MS-MS
MALDI-TOF-TOF MS-MS 中蛋白质的鉴定和结构解析
- 批准号:
7999476 - 财政年份:2010
- 资助金额:
$ 5万 - 项目类别: