ISOLATION OF NEURONAL VOLTAGE SENSITIVE CALCIUM CHANNELS

描述：（改编自申请人的摘要）长期 本申请的目的是阐明本发明化合物的结构和功能， 神经元电压敏感性钙通道（VSCC）的多样性， 通道蛋白的分子克隆和表达。 可用性 神经元VSCC的纯亚型对于高度恶性VSCC的发展至关重要。 选择性拮抗剂具有治疗各种疾病的巨大潜力， 从癫痫到老年痴呆的神经系统疾病。 的 该策略涉及分离结合的通道蛋白， 芋螺毒素，使用包括凝集素的既定程序 层析和亲和层析。 纯化的蛋白质， 已通过亲和交联和光标记鉴定， 通过SDS凝胶电泳分离后电洗脱并测序。 基于肽序列的寡核苷酸探针将用于 筛选电鳐电器官cDNA文库， 并分离cDNA克隆。

DESCRIPTION: (Adapted from the applicant's abstract) The long term objective of this application is to elucidate the structural and functional diversity of neuronal voltage-sensitive calcium channels (VSCC) by molecular cloning and expression of the channel proteins. Availability of pure subtypes of neuronal VSCC is crucial to the development of highly selective antagonists which have great potential for therapy of various neurological diseases ranging from epilepsy to senile dementia. The strategy involves the isolation of the channel protein which binds conotoxin by the use of established procedures involving lectin chromatography and affinity chromatography. The purified protein, which has been identified by affinity cross-linking and photo-labeling, will be electroeluted after separation by SDS gel electrophoresis and sequenced. Oligonucleotide probes based on the peptide sequences will be used to screen a cDNA library of the electric organ of the ray Narcine brasiliensis and isolate cDNA clones.