IDENTIFICATION OF HUMAN F CELL GROWTH FACTOR

胎儿血红蛋白可以大大减轻血红蛋白紊乱的症状 例如影响数百万人镰状细胞性贫血和b地中海贫血 世界各地的人。细胞毒剂目前正被用来尝试 将成人的血红蛋白合成程序转换为胎儿血红蛋白 这些病人。显然有必要开发无毒的药剂来 实现这一结果。也有强有力的证据表明，自然因素 存在能够在体外系统中实现这一点的。我们建议 确定这样的一个或多个因素，我们称之为F细胞因素 (FCF)，使用一种新的两相液体培养系统。在这个化验系统中 我们将不仅检测小牛的血清，这已经被证明是 含有这样一种因子，还包括人脐血血清，患者的血清 他们显示胎儿血红蛋白升高，细胞系的条件培养液， 非人灵长类血清和重组造血生长因子。这 项目将建立可靠的检测方法，L引领未来 该因子的纯化和克隆。

Fetal hemoglobin can greatly reduce the symptoms of hemoglobin disorders such as sickle cell anemia and b-thalassemia diseases which affect millions of people worldwide. Cytotoxic agents are currently being used to attempt to switch the adult program of hemoglobin synthesis to fetal hemoglobin in these patients. There is a clear need to develop nontoxic agents to accomplish this result. There is also strong evidence that natural factors exist which are capable of accomplishing this in vitro systems. We propose to identify such a factor or factors, which we are calling F Cell Factor (FCF), using a new, two phase liquid culture system. In this assay system we will test not only sera from fetal calves, which has been shown to contain such a factor, but also human cord blood sera, serum from patients who show elevated fetal hemoglobulin, conditioned media from cell lines, non-human primate serum and recombinant hemopoietic growth factors. This project will establish a reliable assay and wi]l lead the way for future purification and cloning of the factor.