IDENTIFICATION OF HUMAN F CELL GROWTH FACTOR

人 F 细胞生长因子的鉴定

• 批准号: 3502258
• 负责人: W D HANKINS
• 金额: $ 5万
• 依托单位: MERRIFIELD LABORATORIES, INC.
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-05-01 至 1993-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3502258
• 关键词: RNase protection assay; cell differentiation; cell growth regulation; colony stimulating factor; embryo /fetus cell /tissue; erythroid stem cell; erythropoietin; growth media; hematopoiesis; hematopoietic growth factor; hemoglobin F; human tissue; immunodiffusion; insulin; interferons; interleukin 3; interleukin 6; iron; messenger RNA; molecular cloning; polymerase chain reaction; protein purification; selenium; serum; sickle cell anemia; thalassemia; tissue /cell culture; transferrin; tumor necrosis factor alpha; tumor necrosis factor beta

Fetal hemoglobin can greatly reduce the symptoms of hemoglobin disorders such as sickle cell anemia and b-thalassemia diseases which affect millions of people worldwide. Cytotoxic agents are currently being used to attempt to switch the adult program of hemoglobin synthesis to fetal hemoglobin in these patients. There is a clear need to develop nontoxic agents to accomplish this result. There is also strong evidence that natural factors exist which are capable of accomplishing this in vitro systems. We propose to identify such a factor or factors, which we are calling F Cell Factor (FCF), using a new, two phase liquid culture system. In this assay system we will test not only sera from fetal calves, which has been shown to contain such a factor, but also human cord blood sera, serum from patients who show elevated fetal hemoglobulin, conditioned media from cell lines, non-human primate serum and recombinant hemopoietic growth factors. This project will establish a reliable assay and wi]l lead the way for future purification and cloning of the factor.

胎儿血红蛋白可以大大减轻血红蛋白紊乱的症状 例如影响数百万人镰状细胞性贫血和B-地中海贫血疾病 世界各地的人们。 细胞毒性剂目前正被用来尝试 将成人的血红蛋白合成程序转换为胎儿的血红蛋白， 这些病人。 显然需要开发无毒剂， 实现这个结果。 也有强有力的证据表明自然因素 存在能够实现这种体外系统的方法。 我们提出 来鉴定这样一种因子，我们称之为F细胞因子 (FCF)使用新的两相液体培养系统。 在本试验系统中 我们将不仅测试来自胎牛的血清， 含有这种因子的，还有人的脐带血血清，病人的血清 显示胎儿血红蛋白升高，细胞系的条件培养基， 非人灵长类动物血清和重组造血生长因子。 这 该项目将建立一个可靠的分析，并将引领未来的道路 该因子的纯化和克隆。