ALL SOLID-STATE LASER FOR BIOMEDICAL INSTRUMENTATION

用于生物医学仪器的全固态激光器

• 批准号: 3505511
• 负责人: THOMAS R STEELE
• 金额: $ 5万
• 依托单位: LIGHTWAVE ELECTRONICS CORPORATION
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-09-01 至 1994-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3505511
• 关键词: biomedical device power system; biomedical equipment development; diagnosis quality /standard; fluorescent dye /probe; lasers; semiconduction

Numerous biomedical analysis techniques, such as flow cytometry and confocal microscopy, utilize laser-induced-fluorescence detection. This provides a high sensitivity analysis tool for biomedical research and clinical diagnosis. The most commonly used laser for these applications is the 488nm Ar-ion laser, since it is well matched to the excitation wavelength of fluorescein dyes which are the most widely used. However this laser is less than optimal for these applications: it is fairly large and has low efficiency, so it dissipates much excess heat in the instrument and consumes substantial electrical power. It also has limited lifetime, so regular repair is necessary. This Phase I aims to investigate the feasibility of developing a compact, efficient, robust, long-lifetime laser source that would be a superior, cost-effective replacement for Ar-ion lasers in biomedical instrumentation.It would be based on new InGaAs frequency-doubled in a nonlinear crystal to achieve a wavelength of approximately 490nm, optimal for these applications. We propose to use a resonant doubling cavity to achieve good conversion efficiency. The Phase II follow-on would entail developing a 490nm source optimized for use in biomedical instruments.

许多生物医学分析技术，如流式细胞术和流式细胞仪， 共聚焦显微镜，利用激光诱导荧光检测。 这 为生物医学研究提供了高灵敏度的分析工具， 临床诊断 这些应用中最常用的激光器 是488nm Ar离子激光，因为它与激发很好地匹配， 最广泛使用的荧光素染料的波长。 然而 这种激光器对于这些应用不是最佳的：它相当大 而且效率低，所以它在空气中散发了很多多余的热量， 仪器和消耗大量的电力。 它也有有限的 因此，定期维修是必要的。 第一阶段的目的是研究制定一项契约的可行性， 高效、坚固、长寿命的激光源将是一种上级， 生物医学中Ar离子激光器的经济有效的替代品 它将基于新的InGaAs倍频， 非线性晶体以实现约490nm的波长，最佳 对于这些应用程序。 我们建议使用共振倍频腔， 实现良好的转换效率。第二阶段的后续行动将需要 开发一个490nm的源优化用于生物医学仪器。