NEW FLOW CYTOMETRY TECHNOLOGIES FOR CLINICAL HEMATOLOGY

用于临床血液学的新型流式细胞术技术

• 批准号: 2222113
• 负责人: ROBERT T KUNG
• 金额: $ 28.75万
• 依托单位: ABIOMED, INC.
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-08-15 至 1995-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2222113
• 关键词: bioengineering /biomedical engineering; biomedical equipment development; blood chemistry; cell morphology; clinical biomedical equipment; erythrocyte count; erythrocytes; flow cytometry; fluorescence; fluorescent dye /probe; granulocyte; hematology; hemoglobin; ionophores; lasers; leukocyte count; light scattering; lymphocyte; method development; oscillography; personal computers; photomultiplier; single cell analysis

Automated blood cell analysis is a routine function in the clinical hematology laboratory. We have all had samples taken for such a purpose, but very few realize that the flow cytometric instrumentation performing these tests may have fundamental limitations regarding measurement of red blood cell volume, red blood cell hemoglobin concentration, and accurate classification of white blood cell subpopulations. 'High-end' instruments have become very complex and expensive in order to 'ensure' that an accurate and reliable result can be reported. There remains a gap in the availability of a low cost, easy to use system which can provide accurate and reliable cell data. This proposal describes two novel techniques applicable in clinical flow cytometry for hematologic analysis. Problems commonly associated with hemoglobin concentration measurements and cell volume interferences when making red blood cell measurements and problems of reproducibility of light scattering and volume interferences in the white blood cell differential measurement can be avoided. This is done by a new biomedical instrument that determines cell dry mass by measurements of phase shift using a two-frequency HeNe laser and cell volume by the principle of dye exclusion. The ultimate instrument will provide a unique capability as a low cost device for making accurate red and white blood cell dry mass and volume measurements in single cell analysis.

自动血细胞分析是临床中的常规功能， 血液学实验室我们都曾为此采集过样本， 但很少有人意识到， 这些测试对于红色的测量可能具有基本的局限性 血细胞体积、红细胞血红蛋白浓度， 白色血细胞亚群的分类。 “高端”仪器 已经变得非常复杂和昂贵，以“确保”， 可以报告准确和可靠的结果。在这方面仍然存在差距。 低成本、易于使用的系统的可用性，该系统可以提供准确的 可靠的细胞数据。 该提案描述了两种适用于临床流程的新技术 用于血液学分析的流式细胞术。 通常与以下方面有关的问题 血红蛋白浓度测量和细胞体积干扰， 进行红细胞测量和测量的可重复性问题 白色血细胞中的光散射和体积干扰 可以避免差分测量。 这是由一种新的生物医学 通过测量相移来确定细胞干质量的仪器 利用双频HeNe激光器和细胞容积染料原理 排斥。 最终的仪器将提供一个独特的能力，作为一个低成本 精确测定红、白色血细胞干质量和体积的装置 单细胞分析中的测量。