CANCER, LIPID AND STRESS RESEARCH

该计划旨在激励少数民族教师 成员从事生物医学研究，加强 机构培养研究生和保健学生能力 专业学习，为少数民族学生提供机会， 获得生物医学研究经验，并感兴趣的学生在 从事生物医学科学的研究工作。 为此目的， 该方案包括的次级项目有： 1)脂肪分解酶及其底物 胰腺、小肠和肾脏。 比较 胰腺内源性脂质变化的研究 肠和肾脏的豚鼠和大鼠通过 检查它们各自的脂解能力， 通过体外培养的产物。 的脂质分析 对照品和孵育样品的氯仿：甲醇浸提液 通过多种形式的薄层色谱将提供 数据的基础。 关联相应的TLC观察结果 动情周期相关血浆酶原变化，肾 标本将用于缩醛磷脂组织化学。 2)压力相关的检测与控制 生理变量。 四个变量的调查 （外周温度、脉搏传导时间、心率和皮肤） 决定人类辨别能力的 并主动控制某些生理功能 压力引起的或心身疾病。 检测和 控制是生物反馈中行为方法的基础 训练、整体治疗、冥想技巧和压力 管理 3)铂-胸腺嘧啶联合化疗 癌细胞。 超微结构和组织化学研究 铂-胸腺嘧啶和博莱霉素的联合应用 使用腹水肉瘤-180细胞在体外和体内。 剂量 将监测联合治疗的限制性副作用 在瑞士的肠和肾的癌细胞和正常细胞 白化病小鼠。 联合药物的作用方式为 通过观察它们对碱性磷酸酶的影响， 磷酸酶和钙激活的ATP酶。

The proposed program is designed to motivate minority faculty members to engage in biomedical research, strengthen the institution's capabilities to train students for graduate and health professional study, provide opportunities for minority students to gain biomedical research experience, and interest students in pursing research careers in the biomedical sciences. To this end, the subprojects included in the program are: 1) LIPOLYTIC ENZYMES AND THEIR SUBSTRATES OF PANCREAS, SMALL INTESTINE, AND KIDNEY. A comparative study of the variety of endogenous lipids in the pancreas, small intestine, and kidney of the guinea pig and rat through examination of their respective lipolytic capacities as expressed by the products of in vitro incubation. Lipid analysis of chloroform:methanol extracts of control and incubated samples via multiple forms of thin layer chromatography will provide the basis of the data. To correlate the respective TLC observations with estrous cycle related plasmalogen changes, kidney specimens will be used for the plasmalogen histochemistry. 2) DETECTION VERSUS CONTROL OF STRESS RELATED PHYSIOLOGICAL VARIABLES. Investigation of four variables (peripheral temperature, pulse transit time, heart rate, and skin conductance) determining the ability of humans to discriminate and voluntarily control certain physiological functions related to stress-induced or psychosomatic disorders. Both detection and control underlie the behavioral approach used in biofeedback training, holistic healing, meditation techniques, and stress management. 3) PLATINUM-THYMINE IN COMBINATION CHEMOTHERAPY OF CANCER CELLS. Ultrastructural and histochemical studies of the combined application of platinum-thymine and bleomycin using ascites sarcoma-180 cells in vitro and in vivo. Dose limiting side effects using combination therapy will be monitored on cancer and normal cells of the intestine and kidney in Swiss Albino mice. The mode of action of the combined drugs will be determined histochemically by observing their effect on alkaline phosphatase and calcium-activated ATPase.