MBRS PROGRAM AT WAYNE STATE UNIVERSITY

用微量变形细胞裂解物(LAL)试验试剂制备1h 鲜磨碎肉中内毒素的测定。这些值就是 所代表的革兰氏阴性细菌总数 拉尔决定的内毒素。革兰氏阳性细菌的估计数量 是由新鲜肉类中存在的预先确定的比例决定的 革兰氏阴性菌和革兰氏阳性菌。因此，厌氧菌的好氧平板计数 用LAL法可以在1小时内对新鲜绞肉样品进行快速检测。研究 在整个项目的这一阶段中，将重点放在提取 绞碎肉制品中的内毒素及其聚丙烯酰胺凝胶法拆分。 肉源性微生物对肉鸡内毒素影响的初步研究 肉类原产地将启动。 第1部，共5部。

The Limulus amoebocyte lysate (LAL) test reagent is used to make one h determinations of endotoxins in fresh ground meats. These values are then employed to calculate the total number of gram negative bacteria represented by the LAL-determined endotoxin. The estimated numbers of gram positive bacteria are determined by pre-determined ratios that exist in the fresh meats between gram negative bacteria and gram positives. Thus, the aerobic plate count of a fresh ground meat sample can be estimated in one h by use of LAL. Research during this phase of the overall project will focus on methods for extracting endotoxins from ground meats and their resolution by polyacrylamide gel methods. Initial studies on the effect of meat-borne microorganisms on endotoxins of meat origin will be initiated. Part 1 of 5.