FACSTAR-PLUS DUAL LASER FLOW CYTOMETER

宾夕法尼亚大学科学院 医学有许多合作和独立的研究领域 这将受益于新的荧光激活细胞分选仪 （FACStar Plus）。虽然用户兴趣的范围在 拟议项目的多样性，每一个都对以下方面产生重要影响： 了解和治疗疾病，以及提供洞察力， 细胞活化和分化的基本过程。之多样 这些建议表明，需要建立一个高度通用的、 流式细胞仪。 FACStar Plus将提供最大的灵活性和最先进的 目前可用于细胞分选和分析的先进能力 功能协调发展的 拟议的配置将包含两个激光器和七个 允许前向和正交光散射测量的检测器 沿着五个荧光检测器。 氩离子和 氦氖（HeNe）激光系统将为我们提供最具成本- 分析型流式细胞术和细胞分选中的有效灵活性 目前可用。 相干5瓦氩离子激光器具有多谱线 457.9至514.5 nm的可见光输出线和多线UV输出线 从333.6到363.8 nm，400 mW。蓝色488 nm输出线将允许 使用FITC，PE，碘化丙啶和几种可用的选择 串联染料缀合物。363 nm紫外线输出线将允许使用 钙指示剂Indo-1或DNA结合Hoechst 33342和DAPI。 气冷氦氖激光器在633 nm处将提供35 mW的输出。 这将允许使用藻胆蛋白别藻蓝蛋白（APC）和 潜在地使用新的荧光染料家族，例如花青苷-5。 当与惠普提供的强大数据管理功能相结合时， 340工作站和LYSIDE II软件，该设备将提供 多功能性、模块化和开放式设计， 流式细胞术应用。

The scientific community of the University of Pennsylvania School of Medicine has many collaborative and independent areas of investigation which will benefit from the new fluorescence activated cell sorter (FACStar Plus). While the range of user interests is evident in the diversity of the proposed projects, each bears important implications for understanding and treating disease as well as providing insight into basic processes of cell activation and differentiation. The diversity of the proposals is indicative of the need for a highly versatile, state of the art, flow cytometry machine. The FACStar Plus will provide the greatest flexibility and state-of-the art capabilities currently available for both cell sorting and analytical functions. The proposed configuration will contain two lasers and seven detectors allowing forward and orthogonal light scatter measurements along with five fluorescence detectors. The combination argon-ion and helium-neon (HeNe) laser system will provide us with the most cost- effective flexibility in analytical flow cytometry and cell sorting currently available. A Coherent 5 watt argon-ion laser has multiline visible output lines from 457.9 to 514.5 nm and multiline UV output lines from 333.6 to 363.8 nm at 400 mW. The blue 488 nm output line will allow the use of FITC, PE, propidium iodide and a choice of several available tandem dye conjugates. The 363 nm UV output line will allow the use of the calcium indicator Indo-1 or the DNA binding Hoechst 33342 and DAPI. The air-cooled helium-neon laser will provide 35mW output at 633 nm. This will allow the use of the phycobiliprotein allophycocyanin (APC) and potentially the use of a new family of fluorochromes such as cyanin-5. When combined with powerful data management provided by a Hewlett-Packard 340 workstation and the LYSYS II software, this equipment will provide the versatility, modularity and open-ended design necessary for advanced flow cytometric applications.