PHILIPS CM10 TRANSMISSION ELECTRON MICROSCOPE

飞利浦 CM10 透射电子显微镜

• 批准号: 2283868
• 负责人: Robin Lynn Wright
• 金额: $ 19.6万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-08-15 至 1995-02-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2283868
• 关键词: biomedical equipment purchase; biomedical equipment resource; charge coupled device camera; computer data analysis; computer program /software; computer system hardware; image processing; information display; structural biology; transmission electron microscopy

A state-of-the art Philips CM100 transmission electron microscope is requested for shared use by investigators in the Zoology, Genetics, Biochemistry, and Botany Departments. This microscope will provide high contrast and high resolution transmission electron microscopy of specimens, can rapidly product large numbers of focused images, is chromatically corrected to image thick sections without edge-blurring, and is equipped with a eucentric goniometer for stereo images. The microscope will be integrated into the Department of Zoology's computer system via a CCD TV camera to directly transfer images onto computer disk storage and archiving for image analysis and 3-D reconstruction. This instrument will be housed in the Zoology Department, replacing a 20 year- old Philips 300. The EM laboratory has excellent space for sample preparation, including fixation, embedding, sectioning and vacuum evaporating. Day-to-day operations will be overseen by a highly experienced technician who will also assist in sample preparation. The CM100 will be used primarily by 3 labs to pursue ongoing NIH-funded research programs with significant focuses on ultrastructural analysis of cells and cellular interactions. These investigators have years of cumulative electron microscopy experience and various areas of specialized technical expertise. Interactions among the group will foster scientific and technical progress on current and future research projects in areas ranging from membrane structure to neural function. Approximately 1/2 of the microscope usage will be shared by 16 additional laboratories (8 with NIH funding) whose research requires electron microscopy for less extensive projects. The microscope will also be accessible to other investigators for pilot or short-term projects, extending the usefulness of the instrument to the broadest possible constituency. In addition, availability of a reliable, easily accessible, high-resolution transmission electron microscope will contribute to graduate student training, directly benefitting the Interdisciplinary Molecular and Cellular Biology, Developmental Biology and Genetics training grant programs.

最先进的飞利浦CM100传输电子显微镜是 要求调查人员在动物学，遗传学， 生物化学和植物学部门。 该显微镜将提供高度 对比度和高分辨率透射电子显微镜 标本，可以迅速乘产品的大量集中图像，是 在没有边缘的厚度较厚的部分上校正了图像厚度， 并配备了立体声图像的Eucentric Goniiomer。这 显微镜将集成到动物学系的计算机部 通过CCD电视摄像头系统直接将图像传输到计算机磁盘上 存储和归档以进行图像分析和3-D重建。 这 仪器将安置在动物学部门，取代20年 - 旧飞利浦300。EM实验室有良好的样品空间 准备工作，包括固定，嵌入，切片和真空 蒸发。 日常运营将由高度监督 经验丰富的技术人员也将协助样品准备。 CM100将主要由3个实验室使用 重点关注超微结构分析的研究计划 细胞和细胞相互作用。 这些调查人员有多年 累积电子显微镜经验和各个领域 专业技术专长。 小组之间的互动将 在当前和未来的研究上促进科学和技术进步 从膜结构到神经功能的区域的项目。 大约1/2的显微镜使用情况将通过16个附加共享 研究需要电子的实验室（8个带有NIH资金） 显微镜，用于较少广泛的项目。 显微镜也将是 其他调查人员可以访问飞行员或短期项目， 将仪器的实用性扩展到最广泛的可能性 选区。 此外，可靠，容易的可用性 可访问的高分辨率传输电子显微镜将 为研究生培训做出贡献，直接受益 跨学科分子和细胞生物学，发育生物学 和遗传学培训补助计划。