PHILIPS CM10 TRANSMISSION ELECTRON MICROSCOPE

最先进的Philips CM 100透射电子显微镜 要求动物学、遗传学、 生物化学和植物学系。 这台显微镜将提供高 高分辨透射电子显微镜 标本，可以快速产生大量的聚焦图像， 对厚切片进行色彩校正以成像而没有边缘模糊， 并且配备有用于立体图像的同心测角器。的 显微镜将被集成到动物学系的计算机中 通过CCD电视摄像机将图像直接传送到计算机磁盘上的系统 用于图像分析和3D重建的存储和存档。 这 仪器将被安置在动物学系，取代20年- 老飞利浦300 EM实验室有很好的样品空间 制备，包括固定、包埋、切片和真空 蒸发 日常运作将由一个高度 经验丰富的技术人员，也将协助样品制备。 CM 100将主要由3个实验室使用，以继续进行NIH资助的 研究计划，重点是超微结构分析 细胞和细胞间相互作用。 这些调查人员有多年的 累积的电子显微镜经验和 专业技术知识。 小组之间的互动将 促进当前和未来研究的科学和技术进步 从膜结构到神经功能等领域的项目。 大约1/2的显微镜使用量将由另外16个 研究需要电子的实验室（8个由NIH资助） 显微镜用于不太广泛的项目。 显微镜也将是 其他调查人员也可以利用这些信息进行试点或短期项目， 尽可能广泛地扩大文书的用途 选区 此外，可靠、方便的 高分辨率透射电子显微镜将 有助于研究生培养，直接受益于 跨学科分子与细胞生物学、发育生物学 和遗传学培训资助项目

A state-of-the art Philips CM100 transmission electron microscope is requested for shared use by investigators in the Zoology, Genetics, Biochemistry, and Botany Departments. This microscope will provide high contrast and high resolution transmission electron microscopy of specimens, can rapidly product large numbers of focused images, is chromatically corrected to image thick sections without edge-blurring, and is equipped with a eucentric goniometer for stereo images. The microscope will be integrated into the Department of Zoology's computer system via a CCD TV camera to directly transfer images onto computer disk storage and archiving for image analysis and 3-D reconstruction. This instrument will be housed in the Zoology Department, replacing a 20 year- old Philips 300. The EM laboratory has excellent space for sample preparation, including fixation, embedding, sectioning and vacuum evaporating. Day-to-day operations will be overseen by a highly experienced technician who will also assist in sample preparation. The CM100 will be used primarily by 3 labs to pursue ongoing NIH-funded research programs with significant focuses on ultrastructural analysis of cells and cellular interactions. These investigators have years of cumulative electron microscopy experience and various areas of specialized technical expertise. Interactions among the group will foster scientific and technical progress on current and future research projects in areas ranging from membrane structure to neural function. Approximately 1/2 of the microscope usage will be shared by 16 additional laboratories (8 with NIH funding) whose research requires electron microscopy for less extensive projects. The microscope will also be accessible to other investigators for pilot or short-term projects, extending the usefulness of the instrument to the broadest possible constituency. In addition, availability of a reliable, easily accessible, high-resolution transmission electron microscope will contribute to graduate student training, directly benefitting the Interdisciplinary Molecular and Cellular Biology, Developmental Biology and Genetics training grant programs.