BIO-IMAGING ANALYZER

我们要求为一种新开发的名为生物成像的仪器提供资金 分析仪(也称为磷成像仪)，可用于 放射性标记分子的检测。像32P这样的同位素， ~(125)I、~(35)S和~(14)C，广泛用于蛋白质研究， 核酸和其他大分子可以用这种方法来检测 乐器。虽然用X射线胶片进行放射自显影已被使用 传统上，对于这样的应用，生物成像分析仪是优越的 这是因为：(1)它比X射线胶片的灵敏度高达100倍；(2)它 平均需要10倍的曝光时间；(3)它拥有更多 更宽的动态范围，用于比较两个差异很大的样本 放射强度高于X光胶片；(4)它提供了机会 以电脑为基础的方法，快速而直接地量化数据；以及 (5)可结合新开发的软件阅读 DNA测序等样本会自动凝胶。 加州理工学院的24个实验室将使用这种生物成像分析仪 由大约300名研究人员组成，包括 博士后研究员、研究生和技术人员。这台仪器 将与多种技术结合使用，包括： 免疫印迹；Northern和Southern印迹；CAT检测；RFLP 分析；DNA测序；二维凝胶分析；磷酸肽 作图；以及聚合酶链式反应技术。在这些不同的应用中，分析仪 将用于：(1)用于数据量化；(2)用于图像处理和 数据的计算机分析；以及(3)数据的自动收集。 鉴于其多用途的能力，生物成像分析仪应该大大 在数量和质量上加强我们的研究工作。

We request funding for a newly developed instrument called a bio-imaging analyzer (also known as a phosphorimager) that can be used for the detection of radioactively labeled molecules. Isotopes such as 32p, 125I, 35S, and 14C, that are widely used in the study of proteins, nucleic acids, and other macromolecules can be detected using this instrument. Although autoradiography with X-ray film has been used traditionally for such applications, the bio-imaging analyzer is superior in that: (1) it is up to 100 times more sensitive than X-ray film; (2) it on average requires 10-fold lower exposure times; (3) it possesses a much broader dynamic range for comparing two samples of greatly different radioactive intensity than X-ray film; (4) it provides the opportunity for rapid and direct quantitation of data by computer-based methods; and (5) it can be used in combination with newly developed software to read samples such as DNA sequencing gels automatically. The bio-imaging analyzer will be used by twenty four labs at Caltech consisting of approximately three hundred researchers, including postdoctoral fellows, graduate students, and technicians. The instrument will be used in combination with a wide variety of techniques, including: immunoblotting; Northern and Southern blotting; CAT assays; RFLP analysis; DNA sequencing; two-dimensional gel analysis; phosphopeptide mapping; and PCR techniques. In these various applications, the analyzer will be used: (1) for quantitation of data; (2) for image processing and computer analysis of data; and (3) for automated collection of data. Given its versatile capabilities, the bio-imaging analyzer should greatly enhance our research efforts, both quantitatively and qualitatively.