BIO-IMAGING ANALYZER

生物成像分析仪

• 批准号: 2283815
• 负责人: William G Dunphy
• 金额: $ 18.6万
• 依托单位: CALIFORNIA INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-04-09 至 1995-04-08
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2283815
• 关键词: automated data processing; biomedical equipment purchase; biomedical equipment resource; computer data analysis; computer program /software; computer system hardware; image processing; radiation detector; radiography

We request funding for a newly developed instrument called a bio-imaging analyzer (also known as a phosphorimager) that can be used for the detection of radioactively labeled molecules. Isotopes such as 32p, 125I, 35S, and 14C, that are widely used in the study of proteins, nucleic acids, and other macromolecules can be detected using this instrument. Although autoradiography with X-ray film has been used traditionally for such applications, the bio-imaging analyzer is superior in that: (1) it is up to 100 times more sensitive than X-ray film; (2) it on average requires 10-fold lower exposure times; (3) it possesses a much broader dynamic range for comparing two samples of greatly different radioactive intensity than X-ray film; (4) it provides the opportunity for rapid and direct quantitation of data by computer-based methods; and (5) it can be used in combination with newly developed software to read samples such as DNA sequencing gels automatically. The bio-imaging analyzer will be used by twenty four labs at Caltech consisting of approximately three hundred researchers, including postdoctoral fellows, graduate students, and technicians. The instrument will be used in combination with a wide variety of techniques, including: immunoblotting; Northern and Southern blotting; CAT assays; RFLP analysis; DNA sequencing; two-dimensional gel analysis; phosphopeptide mapping; and PCR techniques. In these various applications, the analyzer will be used: (1) for quantitation of data; (2) for image processing and computer analysis of data; and (3) for automated collection of data. Given its versatile capabilities, the bio-imaging analyzer should greatly enhance our research efforts, both quantitatively and qualitatively.

我们要求为新开发的工具提供资金，称为生物成像 分析仪（也称为磷酸摄影剂），可用于 检测放射性标记的分子。 同位素，例如32p， 125i，35s和14c，在蛋白质研究中广泛使用， 可以使用此检测核酸和其他大分子 乐器。 尽管使用了X射线胶片放射自显影 传统上，对于此类应用，生物成像分析仪是优越的 在那：（1）它比X射线胶片高100倍； （2） 平均需要降低10倍的曝光时间； （3）它拥有很多 更广泛的动态范围，用于比较两个大大不同的样本 放射性强度比X射线膜； （4）它提供了机会 通过基于计算机的方法快速和直接定量数据；和 （5）可以与新开发的软件结合使用它来阅读 诸如DNA测序凝胶之类的样品会自动。 Caltech的二十四个实验室将使用生物成像分析仪 由大约三百位研究人员组成，包括 博士后研究员，研究生和技术人员。 乐器 将与多种技术结合使用，包括： 免疫印迹；北部和南部印迹；猫测定； RFLP 分析; DNA测序；二维凝胶分析；磷酸肽 映射；和PCR技术。 在这些各种应用中，分析仪 将使用：（1）用于定量数据； （2）图像处理和 数据分析； （3）用于自动化数据的收集。 鉴于其多功能功能，生物成像分析仪应大大 在定量和定性上加强我们的研究工作。