BIO-IMAGING ANALYZER

我们申请资助一种新开发的仪器叫做生物成像 分析仪（也称为磷光成像仪），可用于 放射性标记分子的检测。 同位素如32 p， 125 I、35 S和14 C，它们被广泛用于蛋白质的研究， 核酸和其他大分子可以使用这种方法检测。 仪器 虽然放射自显影与X射线胶片已被使用 传统上，对于这些应用，生物成像分析仪是上级的 因为：（1）它比X射线胶片灵敏100倍;（2）它 平均需要低10倍的曝光时间;（3）它具有更大的 更宽的动态范围，用于比较两个差异很大的样品 放射性强度比X线片;（4）它提供了机会 通过基于计算机的方法对数据进行快速和直接的定量;以及 (5)它可以与新开发的软件结合使用， 样品，如DNA测序凝胶自动。 生物成像分析仪将被加州理工学院的24个实验室使用 由大约300名研究人员组成，其中包括 博士后研究员、研究生和技术人员。 仪器 将与各种技术结合使用，包括： 免疫印迹;北方和南方印迹; CAT测定; RFLP 分析; DNA测序;双向凝胶分析;磷酸肽 映射;和PCR技术。 在这些不同的应用中，分析仪 将用于：（1）数据定量;（2）图像处理， 数据的计算机分析;以及（3）用于数据的自动收集。 鉴于其多功能性，生物成像分析仪应大大 在数量和质量上加强我们的研究工作。

We request funding for a newly developed instrument called a bio-imaging analyzer (also known as a phosphorimager) that can be used for the detection of radioactively labeled molecules. Isotopes such as 32p, 125I, 35S, and 14C, that are widely used in the study of proteins, nucleic acids, and other macromolecules can be detected using this instrument. Although autoradiography with X-ray film has been used traditionally for such applications, the bio-imaging analyzer is superior in that: (1) it is up to 100 times more sensitive than X-ray film; (2) it on average requires 10-fold lower exposure times; (3) it possesses a much broader dynamic range for comparing two samples of greatly different radioactive intensity than X-ray film; (4) it provides the opportunity for rapid and direct quantitation of data by computer-based methods; and (5) it can be used in combination with newly developed software to read samples such as DNA sequencing gels automatically. The bio-imaging analyzer will be used by twenty four labs at Caltech consisting of approximately three hundred researchers, including postdoctoral fellows, graduate students, and technicians. The instrument will be used in combination with a wide variety of techniques, including: immunoblotting; Northern and Southern blotting; CAT assays; RFLP analysis; DNA sequencing; two-dimensional gel analysis; phosphopeptide mapping; and PCR techniques. In these various applications, the analyzer will be used: (1) for quantitation of data; (2) for image processing and computer analysis of data; and (3) for automated collection of data. Given its versatile capabilities, the bio-imaging analyzer should greatly enhance our research efforts, both quantitatively and qualitatively.