CONFOCAL SCANNING LASER MICROSCOPE

这项请求是关于Bio-Rad共焦扫描激光显微镜系统的。 它将与尼康Optiphot显微镜配合使用 已经在系里安装了电子显微镜的荧光光学系统。的 俄亥俄州医学院的解剖学。该系统将显著地 加强对十大用户的研究计划，其中七个是 进行国家卫生研究院资助的研究项目。显微镜系统将 还可以由其他四个用户使用，其中三个用户将在 美国国立卫生研究院资助的研究。显微镜系统将用于 几种截然不同的方式。罗迪斯博士将主要用它来 荧光标记初级传入轴突在大鼠脑内的重建 胎鼠。Mooney博士、Chiaia博士和Dun博士将利用它来协助 双侧脑内荧光黄填充神经元的重建 体外和“死片”制剂。邓博士和戈弗雷博士将雇用 重建发射器分布的显微镜系统和 神经组织中与递质相关的酶。莱恩博士，梅尔格伦博士， 莫尔斯、巴德、潘斯基、塞尔曼和查克拉博蒂将使用显微镜 以帮助确定识别抗原的亚细胞位置 多克隆和单克隆抗体，沃尔博士将在 以反射光模式辅助中央重建 辣根过氧化物酶标记的周围神经在 正常和神经受损的猴子。戈德布拉特博士将使用该系统 光动力学肿瘤治疗新药的本地化研究 并评估这种疗法对血管形成的影响。 肿瘤。Bio-Rad系统是最好的仪器 前款规定的申请。调查人员 该应用程序的项目已对这些系统进行了评估 由Bio-Rad、Sarstro和Zeiss开发， Bio-Rad共聚焦显微镜优于其他两种系统。这个 应用程序中包含的初步数据是使用 生物射线显微镜。俄亥俄州医学院有一个强大的 对获得和维护这一目标的机构承诺 显微镜系统。她是一名经验丰富的国家资助的技术人员。 Patricia McCann将监督显微镜的日常操作 还有一间在系里的房间。解剖学部将进行翻新，以容纳该系统。 医学院还同意支付服务合同， 仪器，如果它是购买的。

This request is for a Bio-Rad Confocal Scanning Laser Microscope System. It will be used in conjunction with a Nikon Optiphot microscope equipped with episcopic fluorescence optics already in place in the Dept. of Anatomy at the Medical College of Ohio. The system will significantly enhance the research programs of ten major users, seven of whom are conducting N.I.H.-funded research projects. The microscope system will also be employed by four other users, three of whom will use it in N.I.H.-supported research. The microscope system will be employed in several distinctly different ways. Dr. Rhoades will use it primarily for the reconstruction of fluorescently-labelled primary afferent axons in fetal rats. Drs. Mooney, Chiaia, and Dun will employ it to assist in the reconstruction of neurons that are filled with Lucifer yellow in both in vitro and "dead-slice" preparations. Drs. Dun and Godfrey will employ the microscope system to reconstruct distributions of transmitters and transmitter-related enzymes in neural tissue. Drs. Lane, Mellgren, Morse, Budd, Pansky, Selman, and Chakraborty will employ the microscope to assist in defining the subcellular locations of antigens recognized by polyclonal and monoclonal antibodies and Dr. Wall will use it in the reflected light mode to assist in the reconstruction of the central terminations of horseradish peroxidase-labelled peripheral nerves in normal and nerve-damaged monkeys. Dr. Goldblatt will use the system for localization of new agents being developed for photodynamic tumor therapy and to assess the effects of such therapy upon the vascularization of tumors. The Bio-Rad system is-the best instrument available for the applications outlined in the preceding paragraph. The investigators whose projects comprise this application have evaluated the systems developed by Bio-Rad, Sarastro, and Zeiss and the performance of the Bio-Rad confocal microscope was superior to the other two systems. The preliminary data included in the application were obtained using the Bio-Rad microscope. The Medical College of Ohio has a strong institutional commitment to the acquisition and maintenance of this microscope system. A highly experienced and state-funded technician, Ms. Patricia McCann, will oversee the day-to-day operation of the microscope and a room in the Dept. of Anatomy will be renovated to house the system. The Medical College has also agreed to pay the service contract for the instrument if it is purchased.