AREA DETECTOR-ROTATING ANODE FOR PROTEIN CRYSTALLOGRAPHY

用于蛋白质晶体学的区域检测器旋转阳极

• 批准号: 3521409
• 负责人: JUNG JA P. KIM
• 金额: $ 20.6万
• 依托单位: MEDICAL COLLEGE OF WISCONSIN
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-09-01 至 1993-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3521409
• 关键词: X ray crystallography; biomedical equipment purchase; computer data analysis; computer program /software; computer system hardware; enzyme mechanism; enzyme structure; protein structure

Protein crystallography plays a central role in the advances of biochemical research. This application proposes to set up a state-of-the-art laboratory based X-ray data collection system consisting of an area detector and a rotating anode. The majority of the participants are at the Medical College of Wisconsin, three from the University of Wisconsin-Milwaukee, one member each from the University of Wisconsin-Parkside and the University of Texas Health Science Center at San Antonio. They collaborate on a number of projects of fundamental biomedical importance including: 1) structure and mechanism of acyl-CoA dehydrogenases in fatty acid oxidation, 2) structure of a blue nitrite reductase, 3) mechanism of methyl transfers. in acetyl-CoA synthesis, 4) elucidation of the structural basis for hydroxymethylglutaryl-CoA lyase deficiencies, 5) structure-function studies on a regulated kinase, 6) ATP, ubiquitin-dependent proteolysis, 7) a cellular vaccine against Pseudomonas aeruginosa, 8) structure-function studies on the microsomal flavoprotein, NADPH-cytochrome P450 reductase, 9) structural study of EnvZ, an inner membrane sensor protein in E.coli., and 10) structural studies of a nonamer binding protein in immunoglobulin gene recombination. This facility will allow data to be collected in house at the Medical College of Wisconsin, at a high rate and permits us to expedite the progress and broaden the scope of above projects.

蛋白质晶体学在蛋白质的研究进展中起着核心作用， 生化研究 本申请建议设立一个 最先进的基于实验室的X射线数据采集系统，包括 一个区域探测器和一个旋转阳极。 大多数 参与者在威斯康星州医学院，三个来自 威斯康星大学密尔沃基分校， 威斯康星-帕克赛德和德克萨斯大学健康科学中心在 圣安东尼奥。 他们在一些基础项目上进行合作， 生物医学意义包括：1）酰基辅酶A的结构和机制 脂肪酸氧化中的脂肪酶，2）蓝色亚硝酸盐的结构 还原酶; 3）甲基转移机制。在乙酰辅酶A合成中，4） 羟甲基戊二酰辅酶A裂解酶结构基础的阐明 缺陷，5）调节激酶的结构-功能研究，6） ATP，泛素依赖的蛋白水解，7）细胞疫苗 铜绿假单胞菌，8）微粒体的结构-功能研究 黄素蛋白，NADPH-细胞色素P450还原酶，9）结构研究 EnvZ是大肠杆菌中的内膜传感器蛋白，（10）结构 免疫球蛋白基因九聚体结合蛋白的研究 重组 该设施将允许在医疗中心内部收集数据。 威斯康星州的大学，在一个高利率，并允许我们加快 推进和扩大上述项目的范围。