AREA DETECTOR-ROTATING ANODE FOR PROTEIN CRYSTALLOGRAPHY

蛋白质结晶学在蛋白质的研究进展中起着核心作用。 生化研究。此应用程序建议设置一个 基于实验室的最先进的X射线数据采集系统，包括 由面积探测器和旋转阳极组成。大多数人 参与者来自威斯康星医学院，其中三人来自 威斯康星大学密尔沃基分校，来自密尔沃基大学的一名成员 威斯康星-帕克赛德大学和德克萨斯大学健康科学中心 圣安东尼奥。他们在许多基础项目上进行合作 生物医学意义包括：1)酰基辅酶A的结构和作用机制 脂肪酸氧化中的脱氢酶，2)亚硝酸蓝的结构 还原酶，3)甲基转移机理。在乙酰辅酶A合成中，4) 羟甲基戊二酰辅酶A裂解酶结构基础的阐明 不足之处，5)调控蛋白的结构-功能研究，6) ATP，泛素依赖的蛋白分解，7)一种细胞疫苗 铜绿假单胞菌，8)微粒体的结构-功能研究 黄素蛋白，NADPH-细胞色素P450还原酶，9)结构研究 EnvZ，一种大肠杆菌中的内膜感受器蛋白，以及结构 免疫球蛋白基因中一种非核糖体结合蛋白的研究 重组。 这一设施将允许在医疗中心内部收集数据 威斯康星大学，速度很快，并允许我们加快 取得进展，扩大上述项目的范围。

Protein crystallography plays a central role in the advances of biochemical research. This application proposes to set up a state-of-the-art laboratory based X-ray data collection system consisting of an area detector and a rotating anode. The majority of the participants are at the Medical College of Wisconsin, three from the University of Wisconsin-Milwaukee, one member each from the University of Wisconsin-Parkside and the University of Texas Health Science Center at San Antonio. They collaborate on a number of projects of fundamental biomedical importance including: 1) structure and mechanism of acyl-CoA dehydrogenases in fatty acid oxidation, 2) structure of a blue nitrite reductase, 3) mechanism of methyl transfers. in acetyl-CoA synthesis, 4) elucidation of the structural basis for hydroxymethylglutaryl-CoA lyase deficiencies, 5) structure-function studies on a regulated kinase, 6) ATP, ubiquitin-dependent proteolysis, 7) a cellular vaccine against Pseudomonas aeruginosa, 8) structure-function studies on the microsomal flavoprotein, NADPH-cytochrome P450 reductase, 9) structural study of EnvZ, an inner membrane sensor protein in E.coli., and 10) structural studies of a nonamer binding protein in immunoglobulin gene recombination. This facility will allow data to be collected in house at the Medical College of Wisconsin, at a high rate and permits us to expedite the progress and broaden the scope of above projects.