PROTEIN SEQUENCER SYSTEM

蛋白质测序仪系统

• 批准号: 3521395
• 负责人: EDWARD BRESNICK
• 金额: $ 11万
• 依托单位: DARTMOUTH COLLEGE
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-09-01 至 1993-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3521395
• 关键词: aminoacid analyzer; biomedical equipment purchase; protein purification; protein sequence; synthetic peptide

This Shared Instrumentation Grant Application is for funds to purchase a peptide sequencer for a core laboratory at Dartmouth College and Dartmouth Medical School. The Protein Core Laboratory already contains a peptide synthesizer. Currently, all proteins must be forwarded to another university or industrial concern for sequencing which is expensive and not as efficient as if Dartmouth has its own instrument. A major user group consisting of nationally-funded investigators from the Departments of Biology, Biochemistry, Chemistry, Internal Medicine, Microbiology, Pharmacology and Toxicology, and Physiology has been identified. An Advisory Committee consisting of Drs. Bresnick, Ciardelli and Froehner has been appointed to provide groundrules for the use of the facility, to establish fees for service, and to assist when needed in priority setting for individual tasks (if asked by the Facility Director). The Advisory Committee has appointed Dr. Tom Ciardelli who will have the overall day-to-day responsibility for the protein sequencing service, will oversee the technician, and will establish priorities for proteins to be sequenced. The acquisition of the peptide sequencer will significantly improve the research posture of a group of peer-reviewed investigators and will accordingly, fill a void which is existent on the Dartmouth campus.

此共享工具拨款申请是供基金购买 达特茅斯学院核心实验室的多肽测序仪 达特茅斯医学院。蛋白质核心实验室已经包含了一个 多肽合成器。目前，所有蛋白质都必须转发到 另一所大学或工业界对测序的关注是 价格昂贵，而且效率不如达特茅斯公司有自己的乐器。一个 主要用户群体，由国家资助的来自 生物、生物化学、化学、内科、 微生物学、药理学和毒理学，以及生理学 确认身份。由Ciardelli的Bresnick博士组成的咨询委员会 Froehner已被任命为使用 设施，以确定服务费，并在需要时提供协助 个别任务的优先级设置(如果设施要求 董事)。咨询委员会已任命Tom Ciardelli博士 将全面负责蛋白质的日常工作 排序服务，将监督技术人员，并将建立 对蛋白质进行测序的优先顺序。多肽的获得 Sequencer将显著改善一组 并将相应地填补这一空白，这是 存在于达特茅斯的校园里。