300MHZ NMR SPECTROMETER FOR MEDICINAL CHEMISTRY RESEARCH

用于药物化学研究的 300MHZ 核磁共振波谱仪

• 批准号: 3521512
• 负责人: Vincent Jo Davisson
• 金额: $ 32.2万
• 依托单位: PURDUE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-09-01 至 1993-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3521512
• 关键词: DVD /CD ROM; biomedical equipment purchase; chemistry; computer graphics /printing; computer program /software; computer system hardware; medicine; nuclear magnetic resonance spectroscopy

The Department of Medicinal Chemistry and Pharmacognosy at Purdue University requests funds for the acquisition of a Varian Gemini-300 NMR spectrometer system, with broad-band probes and electronics, variable-temperature capability, and an Ethernet link to a SUN Microsystems SPARC workstation (also requested). The Gemini-300 will replace older and less capable 200 MHz instruments and will allow multinuclear one-dimensional survey spectra (e.g., 1H, 13C, 19F, and 31p) and various two-dimensional experiments to be run routinely, in a "walk-on" mode. The SPARC workstation will also be networked to an existing Varian VXR-500, thus allowing data processing and hard copy production to be accomplished off-line, and maximizing the use of both spectrometers for the acquisition of spectral data. The Major Users' needs for multinuclear observation, variable temperature capability, and an off-line workstation are documented, and a justification for the purchase of a computer-switchable, four-nucleus VT probe is presented. The Major User group comprises 12 faculty members from the Department of Medicinal Chemistry and Pharmacognosy. Dr. Schwab's project concerns the structures and catalytic mechanisms of several bacterial enzymes, and the synthesis of isotopically labeled enzyme substrates and inactivators. Dr. Bergstrom will synthesize and characterize modified oligonucleotides and study their interactions with nucleic acids. Dr. Byrn will synthesize and characterize oligonucleotides tethered to acridines and anthracyclines as potential antiviral agents. Dr. Chang's research concerns the discovery of novel antitumor agents from higher plants, guided by ii variety of mechanism-based bioassays. Dr. Cushman describes the synthesis of a wide range of potential antiviral, antitumor, and antibacterial agents. Dr. Davisson will study enzyme mechanisms in the bacterial pathway for histidine biosynthesis. Dr. Green will synthesize radiopharmaceuticals labeled with metal radionuclides, for use in positron emission tomography (PET). Dr. Loudon is developing new chemical techniques for the identification of peptide hormones in crude biological preparations. Dr. McLaughlin's research centers about the isolation and characterization of novel bioactive principles from higher plants, using several simple and inexpensive, yet effective, bioassays. Dr. Nichols conducts structure-activity studies of dopamine and serotonin agonists and antagonists. Dr. Pidgeon is synthesizing and using NMR spectroscopy to study the structures and dynamics of phospholipids that are covalently linked to silica (immobilized artificial membranes). Dr. Post will use NMR relaxation measurements to probe the active site metal ion environment of phosphoglucomutase. The need for an updated "walk-on" NMR spectrometer is highlighted by the recent growth of the Department's faculty (including five new faculty members over the past two years) as well as the strongly chemical nature of the research being conducted in the Department.

普渡大学药物化学与生药学系 大学申请资金用于购买Varian Gemini-300 NMR 光谱仪系统，具有宽带探头和电子设备， 可变温度能力，以及到SUN的以太网链路 微系统公司的工作站（也要求）。 双子座-300将 更换较旧和功能较弱的200 MHz仪器，并将允许 多核一维测量光谱（例如，1H、13C、19F和31p） 和各种二维实验进行例行，在一个 “跑龙套”模式。 该工作站还将联网到一个 现有的Varian VXR-500，因此允许数据处理和硬拷贝 离线完成生产，并最大限度地利用两者 用于获取光谱数据的光谱仪。 主要用户' 需要多核观测、变温能力， 记录了离线工作站，并说明了 购买计算机可切换，四核VT探头。 主要用户组由12名教师组成，他们来自 药物化学和生药学。 施瓦布博士的项目关注的是 几种细菌酶的结构和催化机制， 同位素标记的酶底物和灭活剂的合成。 博士Bergstrom将合成和表征修饰的寡核苷酸 并研究它们与核酸的相互作用。 伯恩博士将 合成和表征与吖啶连接寡核苷酸， 蒽环类抗生素作为潜在的抗病毒剂。 张博士的研究 涉及从高等植物中发现新的抗肿瘤剂， 通过各种基于机制的生物测定来指导。 库什曼博士描述说， 合成了多种潜在的抗病毒，抗肿瘤， 抗菌剂 戴维森博士将研究酶的机制， 组氨酸生物合成的细菌途径。 绿色博士将合成 用金属放射性核素标记的放射性药物，用于 正电子发射断层扫描（PET）。 劳登博士正在开发新的 原油中肽类激素的化学鉴定技术 生物制剂。 麦克劳克林博士的研究中心 从高等植物中分离和鉴定新生物活性成分 植物，使用几个简单和廉价的，但有效的，生物测定。 博士尼科尔斯进行多巴胺和血清素的结构-活性研究 激动剂和拮抗剂。 皮金博士正在合成和使用核磁共振 光谱学来研究磷脂的结构和动力学， 与二氧化硅共价连接（固定化人工膜）。 博士 Post将使用NMR弛豫测量来探测活性部位金属 磷酸葡萄糖变位酶离子环境。 需要更新的“跑龙套” 核磁共振光谱仪是突出了最近的增长，该部的 教师（包括过去两年的五名新教师）， 以及正在进行的研究的强烈化学性质， 本部门