PROTEIN SEQUENCER/AMINO ACID ANALYZER

蛋白质测序仪/氨基酸分析仪

• 批准号: 3519373
• 负责人: DAVID V COHN
• 金额: $ 18.2万
• 依托单位: UNIVERSITY OF LOUISVILLE
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-04-01 至 1987-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3519373
• 关键词: aminoacid analyzer; enzyme structure; hormone biosynthesis

This application for a Shared Equipment Grant request funds to establish a protein sequencing facility. The users propose to purchase an Applied Biosystems Model 470A gas phase sequencer and a Waters Model 840 parallel-channel HPLC. The latter will be configured to simultaneously analyze PTH-amino acids arising from the Edman degradation sequencing procedure and amino acid compositions of acid hydrolysates of specific proteins and peptides. This equipment will provide a central facility for the analysis and composition of proteins and peptides to support ongoing research activities. The University of Louisville Graduate and Medical Schools have committed long-term funding for maintenance of the instruments and their operation. Research projects that depend on, or will benefit from, the sequencer facility include: analysis of peptide and proteins of the parathyroid gland involved in the processing and secretion of parathormone; study of membrane proteins associated with a human Ca++-pump and comparison to the skeletal and cardiac muscle Ca++-ATPase enzyme partial sequencing of peptides contained within human serum albumin involved in the specific binding of drugs and non-enzymatic glycosylation; characterization of peptides generated from mammalian tumor and synovial collagenases; study of peptides involved in the interactions of cytochrome P-450 and NADPH-P-450 enzymes (Gray); sequencing of S-adenosyl homocysteinase to an extent sufficient to prepare oligonucleotide probes for screening a cDNA library for the gene; a similar project to develop oligonucleotide probes to mammalian "stress" protein genes; structural studies and intercomparisons of unique 34 kDa phosphorylated proteins of mammalian cancer cells related to the binding of epidermal growth factor; structure-activity studies of hormonally active peptides and peptide enzyme inhibitors; and chemical analysis of a new chemotactic factor derived from eosinophils.

本申请的共享设备补助金请求资金建立一个