MASS SPECTROMETRY IN BIOMEDICAL RESEARCH

要求获得支持以获得现代的、更大范围的质量范围 质谱仪，配备了快速原子轰击能力，以及 适用于痕量极性、非挥发性和/或 生物来源的热不稳定的化合物。建议的质量 光谱仪将用于支持美国国立卫生研究院资助的研究项目 华盛顿大学，这需要一个结构特征 多种化合物类型，包括来自蛋白质水解物的多肽， 药物-多肽共价加合物，潜在毒性药物的辅酶A酯 作为环境污染物的代谢物、诱变剂和 异种生物修饰的核酸。这些项目中的每一个都有绝对的 对“软”电离能力的要求以及对具有 高灵敏度的工作质量范围，在1,000-3,000道尔顿范围内 区域。目前还没有这样的质谱学设施，网址为 华盛顿大学。

Support is requested for the acquisition of a modern, extended mass range mass spectrometer, equipped with fast atom bombardment capabilities, and suitable for the analysis of trace amounts of polar, non-volative and/or thermally labile compounds of biological origin. The proposed mass spectrometer will be used in support of NIH-funded research projects at the University of Washington which require structural characterization of a variety of compound types, including peptides from protein hydrolysates, drug-peptide covalent adducts, coenzyme A esters of potentially toxic drug metabolites, mutagenic agents present as environmental contaminants and xenobiotic-modified nucleic acids. Each of these projects has an absolute requirement for "soft" ionization capabilities and for an instrument with an operating mass range, at high sensitivity, in the 1,000-3,000 Dalton region. No such mass spectrometry facilities are currently available at the University of Washington.