MASS SPECTROMETRY IN BIOMEDICAL RESEARCH

生物医学研究中的质谱分析

• 批准号: 3519084
• 负责人: THOMAS A BAILLIE
• 金额: $ 30万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-12-01 至 1985-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3519084
• 关键词: chemical structure; computers; mass spectrometry

Support is requested for the acquisition of a modern, extended mass range mass spectrometer, equipped with fast atom bombardment capabilities, and suitable for the analysis of trace amounts of polar, non-volative and/or thermally labile compounds of biological origin. The proposed mass spectrometer will be used in support of NIH-funded research projects at the University of Washington which require structural characterization of a variety of compound types, including peptides from protein hydrolysates, drug-peptide covalent adducts, coenzyme A esters of potentially toxic drug metabolites, mutagenic agents present as environmental contaminants and xenobiotic-modified nucleic acids. Each of these projects has an absolute requirement for "soft" ionization capabilities and for an instrument with an operating mass range, at high sensitivity, in the 1,000-3,000 Dalton region. No such mass spectrometry facilities are currently available at the University of Washington.

请求提供支助，以购置现代化的、大质量范围的 质谱仪，配备快速原子轰击能力，和 适用于分析痕量的极性、非挥发性和/或 生物来源的热不稳定化合物。 建议的质量 光谱仪将用于支持NIH资助的研究项目， 华盛顿大学，要求结构表征， 多种化合物类型，包括来自蛋白质水解产物的肽， 药物-肽共价加合物，潜在毒性药物的辅酶A酯 代谢物、作为环境污染物存在的诱变剂， 外源性修饰的核酸。 每个项目都有一个绝对的 对“软”电离能力的要求和对具有 在高灵敏度下，工作质量范围为1，000 - 3，000道尔顿 地区 目前没有这样的质谱分析设施， 华盛顿大学。