UPGRADE OF FACS-IV FLOW CYTOMETRY SYSTEM

FACS-IV流式细胞术系统升级

• 批准号: 3519234
• 负责人: FRITZ H BACH
• 金额: $ 10.33万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-12-01 至 1986-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3519234
• 关键词: alopecia; cell differentiation; computer graphics /printing; flow cytometry; fluorescence; fluorescent dye /probe; immunofluorescence technique; immunology; immunotoxicity; lasers; rheumatoid arthritis

The aim of this proposal is to acquire compatible equipment to upgrate an existing flow cytometry and cell sorting facility. The major users of this facility apply flow cytometry and cell sorting to a number of basic research areas, including: cloning of different functional subtypes of human T lymphocytes with anti-tumor and anti-HLA activity; mechanisms of alopecia; differentiation of bone marrow cells; effects of estrogens on breast cancers; depressed immune function in agen; mechanism of rheumatoid arthritis; and effects of immunotoxins on human lymphoid cells. Need for improved instrumentations arises from a number of research aims which can be approached with difficulty, if at all, with the existing equipment. The new equipment requested will be added to the existing FACS-IV flow cytometer and cell sorter. 1) Second argon ion laser and tunable dye head with third fluorescence detection channel. These items will allow three-color immunofluorescence, dual fluorochrome analysis witih forward and 90 degree light scatter, and studies on viable cells requiring cell cycle analysis and immuno-fluorescence. 2) Consort 40C and graphics plotter. The Consort 40C is a high speed, user accessible computer supplied with software to allow acquisition and analysis of multiparameter, list mode data. Without this computer the full capabilities of the existing and requested equipment will not be realized. The graphics plotter is needed to provide legible hard copy output of information processed by the Consort 40C.

本提案的目的是获得兼容设备， 现有的流式细胞术和细胞分选设备。 主要用户 设施应用流式细胞术和细胞分选，以一些基本的 研究领域，包括：克隆不同的功能亚型， 具有抗肿瘤和抗HLA活性人T淋巴细胞; 脱发;骨髓细胞分化;雌激素的作用 乳腺癌;免疫功能低下;类风湿机制 关节炎;以及免疫毒素对人类淋巴细胞的影响。 需要 改进的仪器来自于许多研究目的，这些研究目的可以 如果有的话，用现有的设备很难接近。 的 所要求的新设备将被添加到现有的FACS-IV流程中 流式细胞仪和细胞分选仪。 1)第二氩离子激光器和可调谐染料头 具有第三荧光检测通道。 这些物品将允许 三色免疫荧光，双荧光分析 和90度光散射，以及对需要细胞的活细胞的研究 循环分析和免疫荧光。 2)Consort 40 C和图形 - 是的 Consort 40 C是一款高速、用户可访问的计算机 提供软件以允许采集和分析多参数， 列表模式数据。 如果没有这台计算机， 现有的和要求的设备将无法实现。 图形 需要提供清晰的信息硬拷贝输出 由40 C号配偶处理。