MACROMOLECULAR SYNTHESIS FACILITY

为高分子合成设施申请资金 包括在Applied Biosystems寡核苷酸合成仪上 （型号3808-02），一种Applied Biosystems肽合成仪型号 430 A）和辅助设备，由14个PHS共用 支持研究人员在杜克大学从事24个项目 医学中心 该合成设施的可用性将 加强广泛的基础和临床研究， 学科包括：信号转导（腺苷受体， 趋化性受体，胰腺多肽，T细胞活化， 蛋白激酶C），细胞分化（T细胞成熟，髓样 白血病分化，白细胞介素产生，集落 刺激因子和基质蛋白），病毒学（抗体 产生HTLV，I、II和III型，HTLV III的受体， 允许性与非允许性CMV感染的决定因素）， 代谢（嘌呤代谢错误，AMP调节 脱氨酶，半胱氨酸生物合成的调节）和基因 调控（癌基因，线粒体基因，反义RNA， 珠蛋白、心肌和骨骼肌生长因子和酶 和肺修复）。 寡核苷酸将主要用于 原位杂交组织化学和测序。 合成 肽将用于特异性抗体生产， 识别受体、酶和 基质蛋白 主要用户组估计， 大分子合成设施的最大能力

Funds are requested for a Macromolecular Synthesis Facility comprised on an Applied Biosystems oligonucleotide synthesizer (model 3808-02), an Applied Biosystems peptide synthesizer model 430A) and support equipment, to be shared by a group of 14 PHS supported investigators engaged in 24 projects at Duke University Medical Center. The availability of this synthesis facility will enhance basic and clinical investigation in a wide range of disciplines including: signal transduction (adenosine receptors, chemotactic receptors, pancreatic polypeptides, T-cell activation, protein kinase C), cell differentiation (T-cell maturation, myeloid leukemia differentiation, production of interleukins, colony stimulating factors and matrix proteins), virology (antibody production to HTLV, types I, II and III, receptor for HTLV III, determinants of permissive versus non-permissive CMV infection), metabolism (errors of purine metabolism, regulation of AMP deaminase, regulation of cysteine biosynthesis) and gene regulation (oncogenes, mitochondrial genes, anti-sense RNA, globin, cardiac and skeletal muscle growth factors and enzymes and lung repair). Oligonucleotides will be required primarily for in situ hybridization histochemistry and sequencing. Synthetic peptides will be used for specific antibody production and identification of functional domains in receptors, enzymes and matrix proteins. The major users group estimates that it will use the Macromolecular Synthesis Facility to its full capacity.