REQUEST FOR PROTEIN/PEPTIDE SEQUENCING INSTRUMENTATION

寻求收购蛋白质测序仪器 生物技术中心遗传工程设施 在伊利诺伊大学。 需要这项研究的是 仪器是非常多样化的，反映在范围内， 所描述的研究项目。 几名研究者 需要蛋白质测序仪来解决结构问题 酶的活性位点。 通过位点特异性修饰的残基 可以鉴定作用于酶的试剂。 分析 具有不同的氨基酸序列的单克隆抗体 需要单个半抗原的亲和力来定义组分 决定结合亲和力的抗原结合位点。 结合X射线晶体学数据， 抗体，这些研究将作为未来的基础， 分子建模和基因工程项目， 构建特异性抗体。 活性位点的氨基酸变化是由位点- DNA的定向诱变必须通过测序来确认。 了解蛋白质的N-末端氨基酸序列， 需要肽来鉴定蛋白质的开放阅读框， DNA测序 通常只有少量的蛋白质 可用于这些类型的实验和最先进的 灵敏度在10皮摩尔或更低范围内的仪器 是必需的. 这种蛋白质测序仪的一个独特应用是在 真核细胞中RNA翻译的特异性研究。 该设备在园区集中设施中的存在 将使教师能够进行许多实验，否则， 太昂贵或超出实验室的能力 人员的 它还提供了最有效地利用现有的 设备，避免重复昂贵的仪器， 个别实验室。 生物技术中心和 研究生院将提供实验室空间和充分的 为这些文书的运作提供支助设施。 要去 维修和训练有素的技术人员的存在将 由研究生院保证。

The acquisition of protein sequencing instrumentation is sought for the Genetic Engineering Facility of the Biotechnology Center at the University of Illinois. The research requiring this instrumentation is very diverse as reflected in the range of research projects that are described. Several investigators require the protein sequencer for problems involving the structure of enzyme active sites. The residue modified by site-specific reagents acting on the enzyme can be identified. Analysis of the amino acid sequence of monoclonal antibodies with differing affinities for a single hapten is required to define the components of the antigen binding site which determine the binding affinity. When combined with X-Ray crystallographic data on these antibodies, these studies will serve as the basis for future molecular modeling and genetic engineering programs to construct antibodies of defined specificity. The amino acid changes in active sites which result from site- directed mutagenesis of DNA must be confirmed by sequencing. Knowledge of N-terminal amino acid sequence of proteins and peptides are required to identify open reading frames of sequenced DNA. Often very small amounts of protein are available for these types of experiments and state-of-the-art instrumentation with sensitivity in the range of 10 pmoles or less is required. A unique application of this protein sequencer is in studies on the specificity of RNA translation in eucaryotic cells. Presence of this equipment on campus in a centralized facility will enable faculty to carry out many experiments that otherwise would be too expensive or beyond the capabilities of laboratory personnel. It also provides the most effective use of available equipment and avoids duplication of costly instrumentation in individual laboratories. The Biotechnology Center and the Graduate College will provide the laboratory space and full support facilities for operation of these instruments. On going maintenance and the presence of trained technical personnel will be assured by the Graduate College.