ELECTRON PARAMAGNETIC RESONANCE SPECTROMETER

申请购买电子顺磁仪 共振(EPR)光谱仪提供增强型 宾夕法尼亚州立大学用户的光谱能力 生物科学研究小组。现有的设备已经被 在过去的16年中，被各种实验室广泛使用 几年来，从而建立了一种新的应用范围 来自金属酶(或金属底物酶)的分析 络合物)用于膜和水的物理分析 完整哺乳动物细胞的内环境(S)。这 陈旧的计算机设备及其补充程序 分析，应该更换，因为它的周期性故障现在阻碍 完成实验和/或规范的详细分析。 具体来说，组成申请组的六名调查人员 持有12项目前得到资助的联邦研究拨款，这些拨款利用 电子顺磁共振波谱。主题包括：(A)水的使用 可溶性自旋标记以采样细胞内水的特征，具有 从细胞质的关系到阶段的应用 对许多哺乳动物细胞类型的细胞周期的影响 精子细胞上的冷冻保护剂；(B)使用自旋标记评估 精子中电子传递的独特微扰 与精子表面结合细胞外配体；(C) 精子膜脂类成分的分析 获得生育力；和(D)光谱测定 金属酶的特性，包括锰核苷酸 与绵羊谷氨酰胺合成酶结合的底物络合物， 与真菌木质素酶有关的铁络合物和自由基 与过氧化氢的形成有关。总而言之，一个 预计每周至少使用60个小时以上 为了这件乐器。收到这份文书将保证 在生物化学和细胞生物学方面，这些项目都可以 继续。

Application is made for purchase of an electron paramagnetic resonance (EPR) spectrometer to provide for enhanced spectroscopic capability for a Pennsylvania State University user's group in biological sciences. Existing equipment has been extensively used by a variety of laboratories over the past sixteen years, thereby establishing a pattern of novel applications ranging from analysis of metalloenzymes (or metallosubstrate enzyme complexes) to the physical analysis of the membranes and aqueous internal environment(s) of intact mammalian cells. This outmoded equipment, and its supplemental programs for computer analysis, should be replaced as its periodic failures now hinder completion of experiments and/or detailed analysis of the specta. Specifically, the six investigators forming the applicant group hold twelve currently funded federal research grants that utilize EPR spectroscopy. The topics include: (a) the use of water soluble spin labels to sample features of intracellular water, with applications ranging from relation of the cytoplasm to the stage of the cell cycle for many mammalian cell types to the effect of cryoprotectants on sperm cells; (b) the use of spin labels to assess unique perturbations of electron transport in sperm associated with binding of extracellular ligands to the sperm surface; (c) analysis of the lipid aspects of sperm membranes as related to the acquisition of fertility; and (d) determination of the spectroscopic features of metalloenzymes, including the Mn-nucleotide complexes of the substrates bound to ovine glutamine synthetase, Fe-complexes associated with fungal ligninase, and radicals associated with hydroperoxide formation. In summary, a minimum usage of greater than 60 hours per week is anticipated for this instrument. Receipt of this instrument will assure that these projects, in both biochemistry and cell biology, can continue.