PROTEIN SEQUENCING & ANALYSIS EQUIPMENT

此应用程序是购买设备升级蛋白质 UAB癌症分析和肽合成核心设施 中心 目前可用于识别的仪器 和定量PTH氨基酸，来自Applied Biosystems 气相测序仪和水解产物中的氨基酸不符合 医学院教师的当前需求， 牙科学在速度、灵敏度、定量或可靠性方面。 受影响的具体研究项目，都是由 关于N.I.H.位于U.A.B.的医疗中心，包括 在病毒学（病毒的结构和变异）的广泛领域的研究 流感病毒的抗原位点，逆转录病毒装配过程， 逆转录病毒的免疫抑制机制，包括模型 用于AIDS）、蛋白聚糖结构（蛋白聚糖的功能的定义）、 软骨成分和蛋白聚糖， 动脉粥样硬化中的脂蛋白），蛋白质晶体学 （毒素的作用机制，通过打开或关闭 钠通道，药物设计），免疫学（表征 参与B细胞分化的蛋白质作为标记物 对于肿瘤性B细胞）和基因表达的调节（对照 在E. coli）。 所有这些项目都涉及分析蛋白质， 为了净化，调查人员花费了过多的时间， 钱试图获得足够的蛋白质得到的结果与 大学现有的过时仪器 亚拉巴马对伯明翰。 新设备将大大 提高蛋白质的灵敏度、可靠性和效率 分析和肽合成核心设施，从而提高 亚拉巴马大学NIH资助项目的研究成果 在伯明翰。

This application is to purchase equipment to upgrade the Protein Analysis and peptide Synthesis Core Facility of the UAB Cancer Center. The instrumentation currently available for identification and quantitation of PTH amino acids from the Applied Biosystems Gas-Phase Sequencer and amino acids from hydrolysates does not meet the current needs of faculty in the Schools of Medicine and Dentistry in speed, sensitivity, quantitation, or in reliability. The specific research projects affected, all of which are funded by N.I.H. and located in the Medical Center at U.A.B., include studies in the broad areas of Virology (structure and variation of antigenic sites of influenza virus, retrovirus assembly processes, mechanisms of immunosuppression by retroviruses, including models for AIDS), Proteoglycan Structure (definition of functions of cartilage components and proteoglycans which interact with lipoproteins in atherosclerosis), Protein Crystallography (mechanism of action of toxins which act by opening or closing sodium channels, drug design), immunology (characterization of proteins which are involved in B cell differentiation as markers for neoplastic B cells), and Regulation of Gene Expression (control of pyrimidine biosynthesis in E. coli). All these projects involve analyzing proteins which are difficult to purify, and the investigators are spending excessive time and money trying to obtain sufficient protein to get results with the obsolete instrumentation currently available at the University of Alabama at Birmingham. The new equipment will significantly improve sensitivity, reliability and efficiency of the Protein Analysis and Peptide Synthesis Core Facility, and thus enhance the research output of NIH-funded projects at the University of Alabama at Birmingham.